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Cancer caused by this virus is adult T cell leukemia\/lymphoma which targets 10\u201320 million throughout the world. The effect of this virus extends to the fact that it causes chronic disease to the spinal cord resulting in loss of sensation and further causes blood cancer. So, to overcome the complications, we designed a subunit vaccine by the assimilation of B\u2010cell, cytotoxic T\u2010lymphocyte , and helper T\u2010lymphocyte epitopes. The epitopes were joined together along with adjuvant and linkers and a vaccine was fabricated which was further subjected to 3D modeling. The physiochemical properties, allergenicity, and antigenicity were evaluated. Molecular docking and dynamics were performed with the obtained 3D model against toll like receptor (TLR\u20103) immune receptor. Lastly, in silico cloning was performed to ensure the expression of the designed vaccine in pET28a(+) expression vector. The future prospects of the study entailed the in vitro and in vivo experimental analysis for evaluating the immune response of the designed vaccine construct.<\/jats:p>","DOI":"10.1002\/jcp.27531","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T04:57:30Z","timestamp":1540789050000},"page":"8717-8726","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Combinatorial screening algorithm to engineer multiepitope subunit vaccine targeting human T\u2010lymphotropic virus\u20101 infection"],"prefix":"10.1002","volume":"234","author":[{"given":"Rajan K.","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupal","family":"Ojha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nitesh","family":"Upadhyay","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9401-4400","authenticated-orcid":false,"given":"Amit","family":"Mishra","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Neurobiology Unit, Indian Institute of Technology Jodhpur Jodhpur Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-0596","authenticated-orcid":false,"given":"Vijay K.","family":"Prajapati","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,28]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201009199\u2010w"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-5-76"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.06.080"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201014\u2010S6\u2010S4"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20108\u20104"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2006.06.014"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-890-0:571"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00388"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.13.7568"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki376"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt458"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ct700301q"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00389\u201006"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.085"},{"key":"e_1_2_8_1_16_1","first-page":"1","article-title":"Centers for Disease Control and Prevention and US Public Health Service Working Group for recommendations for counseling persons infected with human T\u2010lymphotrophic virus, types I and II","volume":"42","author":"Khabbaz R. 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