{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:04:27Z","timestamp":1787166267583,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T00:00:00Z","timestamp":1539475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31471355"],"award-info":[{"award-number":["31471355"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Proper left\u2010right (LR) axis establishment is critical for organogenesis in vertebrates. Previously, we reported that zinc finger transcription factors zinc finger transcription factor 1 (\n                    <jats:italic>znfl1s)<\/jats:italic>\n                    are expressed in the tailbud and axial mesoderm in zebrafish. However, a role of\n                    <jats:italic>znfl1s<\/jats:italic>\n                    in LR axis development has not been demonstrated. Here, we discovered that the knockdown of\n                    <jats:italic>znfl1s<\/jats:italic>\n                    using morpholino (MO) in whole embryos or dorsal forerunner cells (DFCs) interrupted LR asymmetry and normal development of the heart, liver, and pancreas. Whole\u2010embryo knockdown of\n                    <jats:italic>znfl1s<\/jats:italic>\n                    by MO or clustered regularly interspaced short palindromic repeat (CRISPR) interference (CRISPRi) resulted in the absent expression of nodal gene\n                    <jats:italic>spaw<\/jats:italic>\n                    and Nodal signaling\u2010related genes\n                    <jats:italic>lft1<\/jats:italic>\n                    ,\n                    <jats:italic>lft2<\/jats:italic>\n                    , and\n                    <jats:italic>pitx2c<\/jats:italic>\n                    in the left lateral plate mesoderm (LPM), and Spaw, Lft1, Lft2, and Pitx2c play important roles in LR axis development in zebrafish. However, specific knockdown of\n                    <jats:italic>znfl1s<\/jats:italic>\n                    in DFCs resulted in random expression of\n                    <jats:italic>spaw<\/jats:italic>\n                    ,\n                    <jats:italic>lft1<\/jats:italic>\n                    ,\n                    <jats:italic>lft2<\/jats:italic>\n                    , and\n                    <jats:italic>pitx2c<\/jats:italic>\n                    . Knockdown of\n                    <jats:italic>znfl1s<\/jats:italic>\n                    led to abnormal cilia formation by the downregulation of\n                    <jats:italic>fgfr1a<\/jats:italic>\n                    and\n                    <jats:italic>foxj1a<\/jats:italic>\n                    expression. The expression of\n                    <jats:italic>spaw<\/jats:italic>\n                    ,\n                    <jats:italic>lft1<\/jats:italic>\n                    ,\n                    <jats:italic>lft2<\/jats:italic>\n                    , and\n                    <jats:italic>pitx2c<\/jats:italic>\n                    was partially rescued by the overexpression of\n                    <jats:italic>fgfr1a<\/jats:italic>\n                    mRNA in\n                    <jats:italic>znfl1s<\/jats:italic>\n                    morphants. Taken together, our results suggest that\n                    <jats:italic>znfl1s<\/jats:italic>\n                    regulate laterality development in zebrafish embryos through controlling the expression of\n                    <jats:italic>fgfr1a<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/jcp.27564","type":"journal-article","created":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T06:53:40Z","timestamp":1539500020000},"page":"1987-1995","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Zebrafish\n                    <i>znfl1s<\/i>\n                    regulate left\u2010right asymmetry patterning through controlling the expression of\n                    <i>fgfr1a<\/i>"],"prefix":"10.1002","volume":"234","author":[{"given":"Jingyun","family":"Li","sequence":"first","affiliation":[{"name":"Maternal and Child Health Medical Institute Women\u2019s Hospital of Nanjing 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