{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T03:17:57Z","timestamp":1773717477427,"version":"3.50.1"},"reference-count":69,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T00:00:00Z","timestamp":1539475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran","award":["IR.TBZMED.REC.1396.1026"],"award-info":[{"award-number":["IR.TBZMED.REC.1396.1026"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Distinct metastasis is one of the main causes of breast cancer (BC)\u2010related mortality and epithelial\u2010mesenchymal transition (EMT) is a primary step in metastasis dissemination. On the other hand, doxorubicin (DOX) is an effective chemotherapeutic agent against BC; unfortunately, its clinical use is limited by dose\u2010dependent side effects. Therefore, extensive efforts have been dedicated to suppressing metastasis of BC and also to overcome DOX side effects together with keeping its antitumor efficacy. Studies supported the role of oleuropein (OLEU) in reducing DOX\u2010induced side effects besides its antitumor actions. In this study, the antimigratory effect of OLEU was assessed and real\u2010time PCR (RT\u2010PCR) was used to detect OLEU effect on the expression level of EMT markers, in MCF\u20107 cells. The cytotoxic effect of OLEU and DOX was assessed by MTT assay, whereas the ratio of apoptosis was investigated by flow cytometry. The results showed that migration ability of MCF\u20107 cells remarkably decreased in OLEU treated group and RT\u2010PCR results showed that OLEU may exert its antimigratory action by suppressing EMT through downregulation of sirtuin1 (SIRT1). Also, the results indicated that both OLEU and DOX were cytotoxic to MCF\u20107 cells, whereas DOX\u2010OLEU cotreatment led to additive cytotoxicity and apoptosis rate. This study provides evidence regarding the suppressive role of OLEU on MCF\u20107 cells migration ability through suppression of EMT, and for the first time, it was proposed that SIRT1 downregulation can be involved in the OLEU antimigratory effect. Also, the findings demonstrated that OLEU can reduce DOX\u2010induced side effects by reducing its effective dose.<\/jats:p>","DOI":"10.1002\/jcp.27586","type":"journal-article","created":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T11:00:02Z","timestamp":1539514802000},"page":"9093-9104","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Oleuropein inhibits migration ability through suppression of epithelial\u2010mesenchymal transition and synergistically enhances doxorubicin\u2010mediated apoptosis in MCF\u20107 cells"],"prefix":"10.1002","volume":"234","author":[{"given":"Jalal","family":"Choupani","sequence":"first","affiliation":[{"name":"Immunology Research Center, Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Medical Genetics Faculty of Medicine, Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5847-3594","authenticated-orcid":false,"given":"Mohammad R.","family":"Alivand","sequence":"additional","affiliation":[{"name":"Immunology Research Center, Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Medical Genetics Faculty of Medicine, Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3398-941X","authenticated-orcid":false,"given":"Sima","family":"M. Derakhshan","sequence":"additional","affiliation":[{"name":"Immunology Research Center, Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Medical Genetics Faculty of Medicine, Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Zaeifizadeh","sequence":"additional","affiliation":[{"name":"Department of Genetic Ardabil Branch Islamic Azad University Ardabil Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4660-0530","authenticated-orcid":false,"given":"Mahmoud","family":"S. Khaniani","sequence":"additional","affiliation":[{"name":"Immunology Research Center, Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Medical Genetics Faculty of Medicine, Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,14]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26754"},{"issue":"1","key":"e_1_2_8_1_3_1","first-page":"31","article-title":"Antiproliferative effect of oleuropein in prostate cell lines","volume":"41","author":"Acquaviva R.","year":"2012","journal-title":"International Journal of Oncology"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.24728"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1370"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.11.048"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.11.065"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/12081679"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.42.1.19"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu8080513"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.612"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.09.072"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201200503"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11127"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000265166"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2016.03.010"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26302"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033183"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2011.09.006"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"crossref","first-page":"380","DOI":"10.12659\/MSM.895312","article-title":"Effect of SIRT1 gene on epithelial\u2010mesenchymal transition of human prostate cancer PC\u20103 cells","volume":"22","author":"Cui Y.","year":"2016","journal-title":"Medical Science Monitor: International Medical Journal of Experimental and Clinical Research"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00853-X"},{"issue":"1","key":"e_1_2_8_1_22_1","first-page":"1175","article-title":"The role of \u03b1\u2010and \u03b2\u2010estrogen receptors on neurite outgrowth in neuro\u20102A cells","volume":"27","author":"Deshotels M. R.","year":"2013","journal-title":"The FASEB Journal"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc745"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.12.009"},{"key":"e_1_2_8_1_25_1","article-title":"Synergistic anti\u2010breast\u2010cancer effects of combined treatment with oleuropein and doxorubicin in vivo","author":"Elamin M. H.","year":"2017","journal-title":"Alternative Therapies in Health and Medicine"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41203"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394311-8.00014-5"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-0935-3"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules200712992"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.8.2197"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-009-9191-2"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2012.13.9.4555"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2013.14.11.6739"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf901280r"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-4231-3"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39104"},{"issue":"11","key":"e_1_2_8_1_37_1","first-page":"797","article-title":"Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L\u2010NAME induced hypertension in rats","volume":"52","author":"Khayyal M. T.","year":"2002","journal-title":"Arzneimittel\u2010Forschung"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.104992"},{"key":"e_1_2_8_1_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002800051097"},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathophys.2015.04.002"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2957-y"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.30"},{"key":"e_1_2_8_1_43_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.4978"},{"key":"e_1_2_8_1_44_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-160471"},{"issue":"1","key":"e_1_2_8_1_45_1","first-page":"129","article-title":"Nobiletin increased cytotoxic activity of doxorubicin on MCF\u20107 cells but not on T47D cells","volume":"3","author":"Meiyanto E.","year":"2011","journal-title":"International Journal of Phytomedicine"},{"key":"e_1_2_8_1_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-010-9178-9"},{"key":"e_1_2_8_1_47_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024369220605"},{"key":"e_1_2_8_1_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1234850"},{"key":"e_1_2_8_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2010.05.005"},{"key":"e_1_2_8_1_50_1","doi-asserted-by":"publisher","DOI":"10.3797\/scipharm.0912-18"},{"key":"e_1_2_8_1_51_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2016.3558"},{"key":"e_1_2_8_1_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.09.029"},{"key":"e_1_2_8_1_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-010-9177-x"},{"key":"e_1_2_8_1_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8281-1"},{"key":"e_1_2_8_1_55_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/927619"},{"key":"e_1_2_8_1_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2016.03.038"},{"key":"e_1_2_8_1_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2014.03.027"},{"key":"e_1_2_8_1_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-008-8014-y"},{"key":"e_1_2_8_1_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1366"},{"key":"e_1_2_8_1_60_1","doi-asserted-by":"publisher","DOI":"10.15171\/apb.2015.049"},{"key":"e_1_2_8_1_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.03.019"},{"key":"e_1_2_8_1_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200900111"},{"issue":"5","key":"e_1_2_8_1_63_1","first-page":"572","article-title":"Olea europaea leaf extract improves the treatment response of GBM stem cells by modulating miRNA expression","volume":"4","author":"Tezcan G.","year":"2014","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_8_1_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001162"},{"key":"e_1_2_8_1_65_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21262"},{"issue":"9","key":"e_1_2_8_1_66_1","first-page":"869","article-title":"Cancer and Mediterranean dietary traditions","volume":"9","author":"Trichopoulou A.","year":"2000","journal-title":"Cancer Epidemiology, Biomarkers & Prevention"},{"key":"e_1_2_8_1_67_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3488-x"},{"key":"e_1_2_8_1_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.05.164"},{"key":"e_1_2_8_1_69_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.8.5.7753"},{"key":"e_1_2_8_1_70_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-13-290"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.27586","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.27586","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.27586","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.27586","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T20:28:00Z","timestamp":1692649680000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.27586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,14]]},"references-count":69,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1002\/jcp.27586"],"URL":"https:\/\/doi.org\/10.1002\/jcp.27586","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,14]]},"assertion":[{"value":"2018-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}