{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:18:18Z","timestamp":1740104298938,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81611540351","81470756","81671005"],"award-info":[{"award-number":["81611540351","81470756","81671005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7172111"],"award-info":[{"award-number":["7172111"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parasympathectomy leads to retrogressive alteration and dysfunction of the submandibular gland (SMG) within 1 month, but its long\u2010term effect is unclear. Excessive secretion is observed in half of the patients 4\u20136 months after SMG transplantation, which completely denervates the gland. Here, we investigated the long\u2010term effect of parasympathectomy on the secretion of SMGs in minipigs. The results showed that the resting salivary secretion of SMGs decreased by 82.9% of that in control at 2 months after denervation, but increased by 156% at 6 months. Although experiencing an atrophic period, the denervated glands regained their normal morphology by 6 months. The expression of the function\u2010related proteins, including muscarinic acetylcholine receptor (mAChR) 3, aquaporin 5 (AQP5), tight junction protein claudin\u20103, and claudin\u20104 was decreased at 2 months after denervation. Meanwhile, the protein expression of stem cell markers, including sex\u2010determining region Y\u2010box 2 and octamer\u2010binding transcription factor 4, and the number of Ki67<jats:sup>+<\/jats:sup> cells were significantly increased. However, at 6 months after denervation, the expression of mAChR3, AQP5, claudin\u20101, claudin\u20103, and claudin\u20104 was significantly raised, and the membrane distribution of these proteins was increased accordingly. The autonomic axonal area of the glands was reduced at 2 months after denervation but returned to the control level at 6 months, suggesting that reinnervation took place in the long term. In summary, parasympathectomy increases resting secretion of the SMGs in the long term with a possible mechanism involving improved transepithelial fluid transport. This finding may provide a new strategy for xerostomia treatment.<\/jats:p>","DOI":"10.1002\/jcp.27640","type":"journal-article","created":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T05:12:28Z","timestamp":1541135548000},"page":"9515-9524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5231-1220","authenticated-orcid":false,"given":"Xue\u2010Ming","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology and Beijing Key Laboratory of Digital Stomatology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology and Beijing Key Laboratory of Digital Stomatology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling\u2010Han","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology and Beijing Key Laboratory of Digital Stomatology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Science Peking University Health Science Center Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang\u2010Yan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology and Beijing Key Laboratory of Digital Stomatology Beijing 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