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Activation of hepatic stellate cell (HSC) is the critical cellular basis for hepatic fibrogenesis, whereas hepatocyte undergoes epithelial\u2010mesenchymal transition (EMT) which is also involved in chronic liver injury. Long noncoding RNA H19 has been found to be associated with cholestatic liver fibrosis lately. However, the role of H19 in liver fibrosis remains largely to be elucidated. In this study, we found that the expression of H19 was significantly upregulated in the liver tissue of CCl<jats:sub>4<\/jats:sub>\u2010induced mice, a toxicant\u2010induced liver fibrogenesis model. Overexpression of H19 significantly aggravated activation of HSC and EMT of hepatocyte both by stimulating transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) pathway. In terms of mechanism, H19 functioned as a competing endogenous RNA to sponge miR\u2010148a and subsequently sustained the level of ubiquitin\u2010specific protease 4 (USP4), which was an identified target of miR\u2010148a and was able to stabilize TGF\u2010\u03b2 receptor I. In conclusion, our findings revealed a novel H19\/miR\u2010148a\/USP4 axis which promoted liver fibrosis via TGF\u2010\u03b2 pathway in both HSC and hepatocyte, indicating that H19 could become a promising target for the treatment of liver fibrosis.<\/jats:p>","DOI":"10.1002\/jcp.27656","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T11:14:33Z","timestamp":1540552473000},"page":"9698-9710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":93,"title":["H19\/miR\u2010148a\/USP4 axis facilitates liver fibrosis by enhancing TGF\u2010\u03b2 signaling in both hepatic stellate cells and hepatocytes"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8943-749X","authenticated-orcid":false,"given":"Jie","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Digestive Diseases Huashan Hospital, Fudan University Shanghai 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