{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:16:24Z","timestamp":1790536584514,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T00:00:00Z","timestamp":1542067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31072103"],"award-info":[{"award-number":["31072103"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81460167"],"award-info":[{"award-number":["81460167"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MicroRNAs (miRNAs) are closely related with the posttranscriptional regulation of gene expression. Our past study showed that let\u20107g\u20105p decreased during pregnancy and lactation in mouse mammary gland, what suggested the prospective regulating role of let\u20107g\u20105p in mammary epithelial cells. In this study, to unravel the role of let\u20107g\u20105p, we found PRKCA (PKC\u2010alpha, PKC\u2010\u03b1) might serve as potential targets of let\u20107g\u20105p by bioinformatics. Then let\u20107g\u20105p was knocked down by an antisense oligonucleotide in mouse primary mammary epithelial cells. As predicted, <jats:italic>PRKCA<\/jats:italic> gene expression and mammary epithelial cells growth were increased by anti\u2010let\u20107g\u20105p regulation in vitro. By using reporter constructs, it showed that the let\u20107g\u20105p could direct binding with 3\u2032\u2010the untranslated regions (3\u2032\u2010UTR) of PRKCA mRNA. Furthermore, ectopic overexpression of let\u20107g\u20105p in vivo reduced PRKCA and \u03b2\u2010casein protein expression as well as inhibited mammary gland growth. These results suggested that let\u20107g\u20105p could inhibit the mammary epithelial cells differentiation and \u03b2\u2010casein protein synthesis and expression through suppression of PRKCA on the cycle of mammary cell differentiation and development\u00a0and that let\u20107g\u20105p may be a novel important regulated target in mammary cells function.<\/jats:p>","DOI":"10.1002\/jcp.27676","type":"journal-article","created":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T14:28:30Z","timestamp":1542119310000},"page":"10101-10110","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Let\u20107g\u20105p regulates mouse mammary cells differentiation and function by targeting PRKCA"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6591-0425","authenticated-orcid":false,"given":"Lei","family":"Tian","sequence":"first","affiliation":[{"name":"Key Laboratory of Dairy Science of Education Ministry, College of Veterinary Medicine, Northeast Agricultural University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science of Education Ministry, College of Veterinary Medicine, Northeast Agricultural University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science of Education Ministry, College of Veterinary Medicine, Northeast Agricultural University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingzhang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science of Education Ministry, College of Veterinary Medicine, Northeast Agricultural University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,13]]},"reference":[{"key":"e_1_2_6_1_2_1","first-page":"4009","article-title":"PKCtheta promotes c\u2010Rel\u2010driven mammary tumorigenesis in mice and humans by repressing estrogen receptor alpha synthesis","volume":"117","author":"Belguise K.","year":"2007","journal-title":"The Journal of Clinical Investigation"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24944"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2014.02.057"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.172"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp857"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707492200"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2184.2003.00265.x"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.3.1026"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.20287"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25927"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.447"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26012"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110190"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.7.3425"},{"key":"e_1_2_6_1_16_1","first-page":"483","article-title":"Expression of let\u20107g\u20105p in development, lactation and involution of the murine mammary gland","volume":"93","author":"Li Y.","year":"2010","journal-title":"Journal of Dairy Science"},{"key":"e_1_2_6_1_17_1","first-page":"239","article-title":"Protein kinase C and mammary cell differentiation: Involvement of protein kinase C alpha in the induction of beta\u2010casein expression","volume":"5","author":"Marte B. 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