{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:55:01Z","timestamp":1770994501629,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,11,22]],"date-time":"2018-11-22T00:00:00Z","timestamp":1542844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ischemia\u2010reperfusion (I\/R) injury often leads to myocardial apoptosis and necrosis. Studies have demonstrated the role microRNAs (miRs) played in myocardial I\/R injury. Thus, we established a myocardial I\/R injury model and a thoracic epidural anesthesia (TEA) model in mice to explore whether microRNA\u2010374 (miR\u2010374) affects myocardial I\/R injury. We collected myocardial tissues to evaluate whether TEA exerts a protection effect on myocardial tissues. In addition, the levels of miR\u2010374, dystrobrevin alpha (DTNA), and the statue of the Notch1 axis were detected. Subsequently, cardiomyocytes extracted from TEA mice were treated to regulate their levels of miR\u2010374 and DTNA. After that, cell viability, cell cycle distribution, and apoptosis of cardiomyocytes were assessed. This was followed by the detection of the myocardial infarction area. The mice models of myocardial I\/R injury were associated with poorly expressed miR\u2010374 and highly expressed DTNA. TEA was found to protect myocardial tissues against myocardial I\/R injury by elevating miR\u2010374 and reducing DTNA. Dual\u2010luciferase reporter assay validated that DTNA was the target gene of miR\u2010374. Cardiomyocytes with overexpressed miR\u2010374 were shown to have downregulated DTNA levels and blocked Notch1 axis. Overexpressed miR\u2010374 was also found to promote the viability and inhibit the apoptosis of cardiomyocytes, as well as to increase the number of cells arrested in the S phase. In accordance with this, the myocardial infarction area was decreased with the upregulated miR\u2010347 and downregulated DTNA. Collectively, these results demonstrated that, by inhibiting the activity of DTNA\u2010mediated Notch1 axis, miR\u2010374 could protect against myocardial I\/R injury in mice after TEA.<\/jats:p>","DOI":"10.1002\/jcp.27745","type":"journal-article","created":{"date-parts":[[2018,12,19]],"date-time":"2018-12-19T15:15:49Z","timestamp":1545232549000},"page":"10726-10740","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Protective role of microRNA\u2010374 against myocardial ischemia\u2010reperfusion injury in mice following thoracic epidural anesthesia by downregulating dystrobrevin alpha\u2010mediated Notch1 axis"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1260-9828","authenticated-orcid":false,"given":"Zheng","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Cardiology Cangzhou Central Hospital Cangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cangzhou People's Hospital Cangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Ying\u2010Chun","sequence":"additional","affiliation":[{"name":"Department of Gynaecology Cangzhou Central Hospital Cangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cangzhou Central Hospital Cangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cangzhou Central Hospital Cangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Guo","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cangzhou Central Hospital Cangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,22]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0528-y"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25218"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.880187"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.5122"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.519405"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jvca.2016.05.039"},{"issue":"2","key":"e_1_2_7_1_8_1","first-page":"566","article-title":"MicroRNA\u2010374a activates Wnt\/beta\u2010catenin signaling to promote breast cancer metastasis","volume":"123","author":"Cai J.","year":"2013","journal-title":"The Journal of Clinical Investigation"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1536\/ihj.16-019"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13752"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3722"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/1658-354X.93048"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060308-103103"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3179"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093213"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI62874"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2011.05.004"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2976"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/genesandcancer.144"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2017.5831"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2010.50"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.4552"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2016.11.009"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2012.09.007"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20170710"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.947531"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i34.6242"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.5773"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038738"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-170592"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms12031660"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-01-00283.2000"},{"key":"e_1_2_7_1_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074315"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgq066"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-013-0373-x"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2011.11.004"},{"key":"e_1_2_7_1_37_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/518926"},{"key":"e_1_2_7_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2014.07.036"},{"key":"e_1_2_7_1_39_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201107083"},{"key":"e_1_2_7_1_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2013.04.014"},{"key":"e_1_2_7_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2014.08.013"},{"key":"e_1_2_7_1_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2210"},{"key":"e_1_2_7_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2011.08.009"},{"key":"e_1_2_7_1_44_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2015.4182"},{"key":"e_1_2_7_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2014.12.027"},{"key":"e_1_2_7_1_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12070"},{"key":"e_1_2_7_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.07.007"},{"key":"e_1_2_7_1_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062652"},{"key":"e_1_2_7_1_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00130.2010"},{"key":"e_1_2_7_1_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-015-1122-4"},{"key":"e_1_2_7_1_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12272"},{"key":"e_1_2_7_1_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2011.03579.x"},{"key":"e_1_2_7_1_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-5454-7"},{"key":"e_1_2_7_1_54_1","doi-asserted-by":"publisher","DOI":"10.1159\/000489186"},{"key":"e_1_2_7_1_55_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-170572"},{"key":"e_1_2_7_1_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8722-6-3"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.27745","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.27745","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.27745","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.27745","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T19:23:26Z","timestamp":1692991406000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.27745"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,22]]},"references-count":55,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1002\/jcp.27745"],"URL":"https:\/\/doi.org\/10.1002\/jcp.27745","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,22]]},"assertion":[{"value":"2018-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}