{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T01:27:35Z","timestamp":1790558855844,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T00:00:00Z","timestamp":1544572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670961"],"award-info":[{"award-number":["81670961"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The rapid bone remodeling after osteotomy has been reported for a long time. However, the underlying mechanism promoting the active bone reconstruction was still to be elucidated. Since not only the bone, blood vessels, and supportive tissues, but also the local microenvironment were destroyed, if the changes on the cell metabolism was contributed to the accelerated bone remodeling came into sight. In present study, we found that the mandibular osteotomy in rabbit activated osteoclasts, as well as the expression of hypoxia\u2010inducible factor 1\u03b1 (HIF\u20101\u03b1) in alveolar bone. Hypoxia or HIF\u20101\u03b1 could enhanced osteoclastogenesis, bone absorption, and lactic acid concentration in receptor activator of nuclear factor \u03ba\u0392 ligand\u2010induced RAW264.7 cells. Coincided with the upregulated HIF\u20101\u03b1 expression, HIF\u2010driven glycolytic enzymes, such as lactate dehydrogenase A (LDHA), glucokinase (GCK), pyruvate kinase M2 (PKM2), and phosphofructokinase1 (PFK1), were found massively increased in both hypoxic RAW264.7 cells and the alveolar HIF\u20101\u03b1\u2010positive osteoclasts after mandibular osteotomy. Knockdown of HIF\u20101\u03b1 suppressed not only the hypoxia\u2010mediated glycolysis, but also the hypoxia\u2010induced acid secretion and bone resorption in RAW264.7 cells. Application of inhibitor on glycolysis gave rise to the similar results as HIF\u20101\u03b1 knockdown. Our findings suggested that hypoxia\u2010driven glycolysis in osteoclasts was an adaptive mechanism to permit alveolar bone remodeling after mandibular osteotomy.<\/jats:p>","DOI":"10.1002\/jcp.27765","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T04:56:18Z","timestamp":1544676978000},"page":"11165-11175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Mandibular osteotomy\u2010induced hypoxia enhances osteoclast activation and acid secretion by increasing glycolysis"],"prefix":"10.1002","volume":"234","author":[{"given":"Yi","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School &amp; Hospital of Stomatology, Tongji University Shanghai China"},{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School &amp; Hospital of Stomatology, Tongji University Shanghai China"},{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danqing","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Jiaotong University First People's Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School &amp; Hospital of Stomatology, Tongji University Shanghai China"},{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School &amp; Hospital of Stomatology, Tongji University Shanghai China"},{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiwen","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School &amp; Hospital of Stomatology, Tongji University Shanghai China"},{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohui","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School &amp; Hospital of Stomatology, Tongji University Shanghai China"},{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoyue","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School &amp; Hospital of Stomatology, Tongji University Shanghai China"},{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zifan","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, School &amp; 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