{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:18:23Z","timestamp":1740104303413,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T00:00:00Z","timestamp":1543276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31702107"],"award-info":[{"award-number":["31702107"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Salmonella<\/jats:italic>Enteritidis (SE) can be transmitted to eggs through cecum or the ovary from infected layers and causes food poisoning in humans. The mechanism of cecal transmission has been extensively studied. However, the mechanism and route of transovarian transmission of SE remain unclear. In this study, the ducks were orally inoculated with SE, and the ovarian follicles and stroma were collected to detect SE infection. The immune responses were triggered and the innate and adaptive immune genes (TLR4, NOD1, Av\u03b2D7, and IL\u20101\u03b2) were upregulated significantly during the SE challenge. Moreover, the ovary tissues (small follicle and stroma) of susceptible and resistant\u2013laying ducks were performed by RNA sequencing. We obtained and identified 23 differentially expressed genes (DEGs) between susceptible and resistant\u2013laying ducks in both small follicle and stroma tissues (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). The DEGs were predominately identified in the p53 signaling pathway. The expression of key genes (p53, MDM2, PERP, caspase\u20103, and Bcl\u20102) involved in the signaling pathway was significantly higher in granulosa cells (dGCs) from SE\u2010infected ducks than those from uninfected ducks. Moreover, the overexpression of PERP resulted in further induction of p53, MDM2, caspase\u20103, and Bcl\u20102 during SE infection in dGCs. Whereas, an opposite trend was observed with the knockdown of PERP. Besides, it is further revealed that the PERP could enhance cell apoptosis, SE adhesion, and SE invasion in SE\u2010infected dGCs overexpression. Altogether, our results demonstrate the duck PERP involved in the ovarian local immune niche through p53 signaling pathway in dGCs challenged with SE.<\/jats:p>","DOI":"10.1002\/jcp.27790","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T09:06:36Z","timestamp":1543309596000},"page":"11330-11347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative transcriptome analysis reveals PERP upregulated during<i>Salmonella<\/i>Enteritidis challenge in laying ducks"],"prefix":"10.1002","volume":"234","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[{"name":"Joint International Research Laboratory of Agriculture &amp; Agri\u2010Product Safety of Ministry of Education, Yangzhou University Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian\u2010tian","family":"Gu","sequence":"additional","affiliation":[{"name":"Joint International Research Laboratory of Agriculture &amp; Agri\u2010Product Safety of Ministry of Education, Yangzhou University Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"Joint International Research Laboratory of Agriculture &amp; Agri\u2010Product Safety of Ministry of Education, Yangzhou University Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Fujian Academy of Agricultural Sciences Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinping","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Hubei Academy of Agricultural Sciences Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizhi","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Zhejiang Academy of Agricultural Sciences Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo\u2010qiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Joint International Research Laboratory of Agriculture &amp; Agri\u2010Product Safety of Ministry of Education, Yangzhou University Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2791-0429","authenticated-orcid":false,"given":"Qi","family":"Xu","sequence":"additional","affiliation":[{"name":"Joint International Research Laboratory of Agriculture &amp; Agri\u2010Product Safety of Ministry of Education, Yangzhou University Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2413-5589","authenticated-orcid":false,"given":"Guo\u2010hong","family":"Chen","sequence":"additional","affiliation":[{"name":"Joint International Research Laboratory of Agriculture &amp; Agri\u2010Product Safety of Ministry of Education, Yangzhou University Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,27]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.6.704"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7.10.986"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/83.6.997"},{"issue":"4","key":"e_1_2_8_1_5_1","doi-asserted-by":"crossref","first-page":"489","DOI":"10.2460\/ajvr.1996.57.04.489","article-title":"Salmonella enteritidis in eggs, cloacal swab specimens, and internal organs of experimentally infected White Leghorn chickens","volume":"57","author":"Bichler L. 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