{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:46:00Z","timestamp":1791888360631,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T00:00:00Z","timestamp":1545782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Applied Medicine Research Program of Hefei City","award":["hwk2017yb005"],"award-info":[{"award-number":["hwk2017yb005"]}]},{"name":"Clinical Key Program of Hefei City Science and Technology Bureau","award":["YW201710120005"],"award-info":[{"award-number":["YW201710120005"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study aims to verify whether the inhibitory effect of Sirtuin 3 (SIRT3) on the formation of renal calcium oxalate crystals was mediated through promoting macrophages (M\u03d5s) polarization. Identification and quantification of M1 and M2 monocytes were performed using fluorescence\u2010activated cell sorting analysis. SIRT3 protein level and forkhead box O1 (FOXO1) acetylation level were measured using western blot analysis. Cell apoptosis of HK\u20102 was detected by flow cytometry. Mouse kidney tissues were subjected to Von Kossa staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and immunohistochemical staining for detection of kidney crystals deposition, apoptosis, and expression of crystal\u2010related molecules, respectively. The results showed that human peripheral blood monocytes from patients with kidney stone (KS) exhibited decreased M2 monocytes percentage and SIRT3 expression, whereas increased FOXO1 acetylation compared with the normal controls. In vitro assay revealed that SIRT3 overexpression in bone marrow\u2010derived M0\/M1\/M2 M\u03d5s induced M2 polarization and decreased FOXO1 acetylation. Furthermore, FOXO1 knockdown reversed SIRT3\u2010mediated induction of M2 polarization and inhibition of HK\u20102 (human proximal tubular cell line) apoptosis. Further in vivo experiments demonstrated that SIRT3\u2010overexpressing M\u03d5s transfusion not only induced M2 polarization, but also alleviated inflammation, apoptosis, and crystals deposition in glyoxylate\u2010induced KS mice. In conclusion, SIRT3 suppresses formation of renal calcium oxalate crystals through promoting M2 polarization via deacetylating FOXO1.<\/jats:p>","DOI":"10.1002\/jcp.27803","type":"journal-article","created":{"date-parts":[[2018,12,27]],"date-time":"2018-12-27T01:34:25Z","timestamp":1545874465000},"page":"11463-11473","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Sirtuin 3 suppresses the formation of renal calcium oxalate crystals through promoting M2 polarization of macrophages"],"prefix":"10.1002","volume":"234","author":[{"given":"Junhua","family":"Xi","sequence":"first","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"},{"name":"Institute of Urology, Anhui Medical University Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University Hefei Anhui China"},{"name":"Department of Urology Hefei Hospital Affiliated to Anhui Medical University (The Second People's Hospital of Hefei) Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"},{"name":"Institute of Urology, Anhui Medical University Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfeng","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Urology Hefei Hospital Affiliated to Anhui Medical University (The Second People's Hospital of Hefei) Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"},{"name":"Institute of Urology, Anhui Medical University Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University Hefei Anhui China"},{"name":"Department of Urology Hefei Hospital Affiliated to Anhui Medical University (The Second People's Hospital of Hefei) Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1624-7409","authenticated-orcid":false,"given":"Chaozhao","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"},{"name":"Institute of Urology, Anhui Medical University Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongyao","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"},{"name":"Institute of Urology, Anhui Medical University Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"},{"name":"Institute of Urology, Anhui Medical University Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,26]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2016.1256884"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12786"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2010070693"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8162"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.3A0514-251R"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.05.028"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2012.01.042"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01958"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014050479"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100800"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000430106"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13429"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201701449R"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838918"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/kisup.2014.7"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-007-0082-8"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.080514"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000444370"},{"key":"e_1_2_7_1_20_1","first-page":"9196","article-title":"Overexpression of Sirt3 inhibits lipid accumulation in macrophages through mitochondrial IDH2 deacetylation","volume":"8","author":"Sheng S.","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.10.002"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-016-0882-9"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.07.083"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35167"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2013060675"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370213493069"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2015-1301"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-013-9520-4"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2017.0517"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.578260"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2015.254"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.27803","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.27803","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.27803","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.27803","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T15:19:11Z","timestamp":1692976751000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.27803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,26]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1002\/jcp.27803"],"URL":"https:\/\/doi.org\/10.1002\/jcp.27803","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,26]]},"assertion":[{"value":"2018-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}