{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:08:31Z","timestamp":1791562111882,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,1,13]],"date-time":"2019-01-13T00:00:00Z","timestamp":1547337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Lianyungang health bureau research project","award":["201510"],"award-info":[{"award-number":["201510"]}]},{"name":"Lianyungang health bureau research project","award":["1402"],"award-info":[{"award-number":["1402"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the role of miR\u2010424 in diabetic nephropathy (DN) and its relationship with Rictor in mammalian target of rapamycin (mTOR) C2\/Akt signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The western blot analysis and real\u2010time polymerase chain reaction were used to determine the differential expression of Rictor, mTOR, and miR\u2010424 in DN rats. The upregulation of miR\u2010424 was achieved by caudal vein injection of miR\u2010424 mimics. The renal lesion was evaluated by hematoxylin\u2010eosin staining (H&amp;E) and periodic acid schiff staining. The dual\u2010luciferase reporter assay was conducted to determine the binding target of miR\u2010424. The effect of miR\u2010424 upregulation on apoptosis was detected by the terminal deoxynucleotidyl transferase\u2010mediated 2\u2010Deoxyuridine\u20105\u2010Triphosphate (dUTP) nick\u2010end labeling assay and western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significantly lower expression of miR\u2010424 and a significantly higher expression of Rictor and mTOR were found in renal tissues of DN rats. The upregulation of miR\u2010424 improved renal lesion and DN symptoms of blood glucose level, urine protein level, body weight, creatinine level, blood urea nitrogen, and KW\/BW ratio. The upregulation of miR\u2010424 could significantly reduce apoptosis rates of tissue cells by decreasing the expression levels of caspase\u20103 and Bax as well as increasing the level of Bcl\u20102. Furthermore, Rictor was the direct target for miR\u2010424, and upregulation of miR\u2010424 inhibited Rictor through Akt signaling in renal tissue of DN rats and high\u2010glucose\u2010treated human glomerular mesangial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>miR\u2010424 contributes to alleviating the symptoms in DN rat models by targeting Rictor through mTORC2\/Akt signaling.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27822","type":"journal-article","created":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T05:05:42Z","timestamp":1547442342000},"page":"11646-11653","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Upregulation of microRNA\u2010424 relieved diabetic nephropathy by targeting Rictor through mTOR Complex2\/Protein Kinase B signaling"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3085-0869","authenticated-orcid":false,"given":"Guofeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of endocrinology The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, The Affiliated Hospital of Kangda College of Nanjing Medical University Lianyungang Clinical College of Nanjing Medical University Lianyungang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxin","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of endocrinology The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, The Affiliated Hospital of Kangda College of Nanjing Medical University Lianyungang Clinical College of Nanjing Medical University Lianyungang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of endocrinology The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, The Affiliated Hospital of Kangda College of Nanjing Medical University Lianyungang Clinical College of Nanjing Medical University Lianyungang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of endocrinology The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, The Affiliated Hospital of Kangda College of Nanjing Medical University Lianyungang Clinical College of Nanjing Medical University Lianyungang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of endocrinology The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, The Affiliated Hospital of Kangda College of Nanjing Medical University Lianyungang Clinical College of Nanjing Medical University Lianyungang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,13]]},"reference":[{"key":"e_1_2_11_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2014.05.011"},{"key":"e_1_2_11_1_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.035592"},{"issue":"5","key":"e_1_2_11_1_4_1","first-page":"1426","article-title":"miR\u2010424 protects PC\u201012 cells from OGD\u2010induced injury by negatively regulating MKP\u20101","volume":"22","author":"Cao Y. 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