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In this study, we addressed the dependence of somatic release on Ca<jats:sup>2+<\/jats:sup> influx in trigeminal neurons and the involvement of the different types of voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> (Cav) channels in the process. Similar to the closely related dorsal root ganglion neurons, we found two kinetically distinct components of somatic release, a faster component stimulated by voltage but independent of the Ca<jats:sup>2+<\/jats:sup> influx, and a slower component triggered by Ca<jats:sup>2+<\/jats:sup> influx. The Ca<jats:sup>2+<\/jats:sup>\u2010dependent component was inhibited 80% by \u03c9\u2010conotoxin\u2010MVIIC, an inhibitor of both N\u2010 and P\/Q\u2010type Cav channels, and 55% by the P\/Q\u2010type selective inhibitor \u03c9\u2010agatoxin\u2010IVA. The selective L\u2010type Ca<jats:sup>2+<\/jats:sup> channel inhibitor nimodipine was instead without effect. These results suggest a major involvement of N\u2010 and P\/Q\u2010, but not L\u2010type Cav channels in the somatic release of trigeminal neurons. Thus antinociceptive Cav channel antagonists acting on the N\u2010 and P\/Q\u2010type channels may exert their function by also modulating the somatic release and cross\u2010communication between sensory neurons.<\/jats:p>","DOI":"10.1002\/jcp.27901","type":"journal-article","created":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T05:27:51Z","timestamp":1544246871000},"page":"10977-10989","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Ca<sup>2+<\/sup>\u2010dependent and Ca<sup>2+<\/sup>\u2010independent somatic release from trigeminal neurons"],"prefix":"10.1002","volume":"234","author":[{"given":"Luigi","family":"Sforna","sequence":"first","affiliation":[{"name":"Department of Chemistry, Biology, and Biotechnology University of Perugia Perugia Italy"},{"name":"Department of Experimental Medicine University of Perugia Perugia 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