{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T23:28:51Z","timestamp":1780702131975,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81501333"],"award-info":[{"award-number":["81501333"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81401218"],"award-info":[{"award-number":["81401218"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31671567"],"award-info":[{"award-number":["31671567"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81401274"],"award-info":[{"award-number":["81401274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81801479"],"award-info":[{"award-number":["81801479"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>AbstractBackground<\/jats:title><jats:p>Monocytic myeloid\u2010derived suppressor cells (MO\u2010MDSCs) play an important role in maintaining normal pregnancy. However, it is still not clear what kind of changes in MO\u2010MDSCs may lead to miscarriage, and which gene expression changes take place when MO\u2010MDSCs migrate to the uterus as bone marrow\u2010derived cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used flow sorting technology to obtain MO\u2010MDSCs from the maternal\u2013fetal interface and bone marrow, respectively. Affymetrix 3\u2032IVT expression profiling chip technology was used to detect the differential gene expression profiles in MO\u2010MDSCs at the maternal\u2013fetal interface in a mouse model of spontaneous abortion compared with the normal fertility control mice. We also compared the differential gene expression of MO\u2010MDSCs at the maternal\u2013fetal interface compared with bone marrow in the normal fertility control mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that 3,409 genes in MO\u2010MDSCs were upregulated and 1,539 genes were downregulated at the maternal\u2013fetal interface in the spontaneous abortion mice compared with the normal fertility mice. These genes are enriched in cellular components, biological processes, molecular functions, and protein binding, tumor signaling pathway, the PI3K\u2013Akt signaling pathway, intratumoral proteoglycans, and extracellular matrix receptor interactions. Furthermore, we found that 270 genes in MO\u2010MDSCs were upregulated and 383 genes were downregulated at the maternal\u2013fetal interface in the normal fertility mice compared with those in the bone marrow. These genes are enriched in cellular components, biological processes, molecular functions, cell cycle, tumor transcriptional disorder, and cell adhesion molecules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Differential gene expression in MO\u2010MDSCs likely contributes to a successful pregnancy in fetal\u2013maternal immunotolerance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27902","type":"journal-article","created":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T06:48:53Z","timestamp":1544770133000},"page":"10789-10799","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Differential gene expression profile in monocytic myeloid\u2010derived suppressor cells at maternal\u2013fetal interface in a mouse model of spontaneous abortion"],"prefix":"10.1002","volume":"234","author":[{"given":"Jiabin","family":"Ren","sequence":"first","affiliation":[{"name":"Department of Obstetrics and 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