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The rats were divided into control (C, <jats:italic>n<\/jats:italic>\u2009=\u20098), diabetic (DM, <jats:italic>n<\/jats:italic>\u2009=\u20098), diabetic treated with systemic RSV (DM\u2010RSV, <jats:italic>n<\/jats:italic>\u2009=\u20098), diabetic treated with RSV and nonconditioned MSCs (DM\u2010RSV\u2010MSCs, <jats:italic>n<\/jats:italic>\u2009=\u20098), diabetic treated with RSV and RSV\u2010incubated with MSCs (DM\u2010RSV\u2010MSCc, <jats:italic>n<\/jats:italic>\u2009=\u20098) and diabetic treated with RSV\u2010conditioned MSCs (DM\u2010MSCc, <jats:italic>n<\/jats:italic>\u2009=\u20098). Echocardiography (Echo) showed significant improvement of cardiac functions in all groups treated with RSV either systemic or added in culture media. Data of ejection fraction (EF%) of DM\u2010RSV\u2010MSCc (81.50; interquartile range [IQR], 80.00\u201383.00) was comparable to both DM\u2010RSV\u2010MSCs (77.50; IQR, 71.50\u201379.00), and DM\u2010MSCc (71.50; IQR, 70.00\u201374.50). Histological examination of the left ventricles was performed for all groups. DM group revealed significant myocardial hypertrophy, apoptosis, interstitial fibrosis, and microvascular affection. All treated groups were associated, in variable degrees, with attenuation of cardiac hypertrophy and fibrosis, decreased area% for cardiac immunostaining of secreted frizzled\u2010related protein (sFRP2) and Wnt\/\u03b2\u2010catenin and improvement of the microvasculature. In conclusion, MSCs pretreated with resveratrol for 7 days showed increased 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide (MTT) assay, and combined use of RSV (systemically and in culture media) significantly could improve cardiac remodeling capacity of MSCs via attenuation of sFRP2\u2010mediated fibrosis and the downstream Wnt\/\u03b2\u2010catenin pathway.<\/jats:p>","DOI":"10.1002\/jcp.27947","type":"journal-article","created":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T05:33:07Z","timestamp":1544247187000},"page":"10942-10963","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Combined treatment with systemic resveratrol and resveratrol preconditioned mesenchymal stem cells, maximizes antifibrotic action in diabetic cardiomyopathy"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4250-0818","authenticated-orcid":false,"given":"Asmaa Mohammed","family":"ShamsEldeen","sequence":"first","affiliation":[{"name":"Department of Physiology Faculty of Medicine, Cairo University Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hend","family":"Ashour","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Medicine, Cairo University Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heba Samy","family":"Shoukry","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Medicine, Cairo University Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mostafa","family":"Fadel","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Imaging and Endoscopy Unit, Animal Reproduction Research Institute Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samaa Samir","family":"Kamar","sequence":"additional","affiliation":[{"name":"Department of Medical Histology Faculty of Medicine, Cairo University Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marwan","family":"Aabdelbaset","sequence":"additional","affiliation":[{"name":"Department of Pharmacology National Research Center Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laila Ahmed","family":"Rashed","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine, Cairo University Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hania Ibrahim","family":"Ammar","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Medicine, Cairo University Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,7]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/db13-0896"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.110.952556"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1734"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0142-x"},{"key":"e_1_2_8_1_6_1","first-page":"1413","article-title":"Transforming growth factor\u2010b\u2010dependent Wnt secretion controls myofibroblast formation and myocardial fibrosis progression in experimental autoimmune myocarditis","volume":"38","author":"Blyszczuk P.","year":"2017","journal-title":"European Heart Journal"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-010-9131-7"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52032-6"},{"issue":"8","key":"e_1_2_8_1_10_1","first-page":"391","article-title":"Biostatistics102: Quantitative data\u2014Parametric & non\u2010parametric tests","volume":"44","author":"Chan Y. 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