{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:25:35Z","timestamp":1787127935309,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,12,7]],"date-time":"2018-12-07T00:00:00Z","timestamp":1544140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,12,7]],"date-time":"2018-12-07T00:00:00Z","timestamp":1544140800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["92\u201001\u201041\u201019444"],"award-info":[{"award-number":["92\u201001\u201041\u201019444"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Impaired wound healing and skin dehydration are the mainstay of systemic sclerosis (SSc) cutaneous manifestations. Aquaporin\u20103 (AQP3) has a pivotal role in skin hydration and wound healing. Epidermal growth factor receptor (EGFR) activation is impaired in SSc fibroblasts. It is unclear whether AQP3 downregulation or epidermal growth factor (EGF) signaling are the primary points of dysregulation in SSc patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Skin punch biopsies were obtained from 10 SSc patients and 10 healthy subjects. The mRNA and\/or protein expression levels of AQP3, EGFR\/p\u2010EGFR, matrix metalloproteinase\u20101\/2\/9 (MMP\u20101\/2\/9), and tissue inhibitors of metalloproteinase\u20101 (TIMP1) at baseline and after EGF and transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) treatment was evaluated in extracted fibroblasts using real\u2010time polymerase chain reaction and western blot analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SSc fibroblasts expressed lower AQP3 and EGFR, compared with normal fibroblasts. Normal fibroblasts increased AQP3 expression in response to EGF whereas AQP3 expression had no change in EGF\u2010treated\u2010SSc fibroblasts. Likewise, EGFR was activated in response to EGF in the normal group but not SSc group. Baseline expression of MMP\u20101\/2\/9 and TIMP1 was not different between SSc and controls. EGF treatment did not result in alteration of any MMPs expression in either of the groups. Combination treatment resulted in a significant upregulation of MMP\u20101 in normal fibroblasts compared with SSc fibroblasts, while in SSc fibroblasts MMP\u20109 expression was upregulated in response to treatment with TGF\u2010\u03b21 only.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Downregulation of AQP3 expression in SSc fibroblasts may be related to reduced EGFR expression and activation. TGF\u2010\u03b21 (alone or in combination with EGF) only can upregulate AQP3 expression in SSc fibroblasts so, TGF\u2010\u03b21 affect MMP\u20101 and MMP\u20109 just in SSc fibroblasts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.27952","type":"journal-article","created":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T00:22:02Z","timestamp":1544228522000},"page":"12876-12883","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Attenuation of aquaporin\u20103 and epidermal growth factor receptor expression and activation in systemic sclerosis dermal fibroblasts"],"prefix":"10.1002","volume":"234","author":[{"given":"Elham","family":"Farhadi","sequence":"first","affiliation":[{"name":"Rheumatology Research Center, Tehran University of Medical Sciences Tehran 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