{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:25:36Z","timestamp":1787127936309,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Long noncoding RNAs (lncRNAs) exert critical roles in the development of various cancers, including human laryngeal cancer. Radioresistance contributes to the predominant causes of laryngeal cancer recurrence after radiotherapy. The aim of our study was to investigate the association of dysregulated lncRNA and radiation resistance in human larynx squamous carcinoma. Here, we investigated the biological roles of lncRNA DiGeorge syndrome critical region gene 5 (DGCR5) in radioresistance of human laryngeal cancer. Two human larynx squamous carcinoma cell lines (Hep\u20102 and Hep\u20102R), with different radiosensitivities in vitro were used in the present study. We observed that DGCR5 was significantly upregulated in Hep\u20102R cells. Inhibition of DGCR5 by LV\u2010shDGCR5 transfection restrained Hep\u20102R cell proliferation and sensitized cells to radiation. Reversely, overexpression of DGCR5 exhibited an opposite phenomenon in vitro. In addition, microRNA (miR)\u2010195 was predicted as a direct downstream target of DGCR5. Dual\u2010luciferase reporter and RNA immunoprecipitation assays verified the direct interaction between them. Meanwhile, miR\u2010195 was observed to be reduced in Hep\u20102R cells and miR\u2010195 mimics repressed Hep\u20102 cell growth. Moreover, radiosensitivity of Hep\u20102R cells was greatly enhanced by overexpression of miR\u2010195, which could be reversed by upregulation of DGCR5. Finally, in vivo experiments were used to validate that knockdown of DGCR5 suppressed laryngeal carcinoma via targeting miR\u2010195. In conclusion, we indicated that DGCR5 could contribute to the radioresistance of human laryngeal carcinoma cells via sponging miR\u2010195.<\/jats:p>","DOI":"10.1002\/jcp.27958","type":"journal-article","created":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T01:43:39Z","timestamp":1544751819000},"page":"12918-12925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Knockdown of DGCR5 enhances the radiosensitivity of human laryngeal carcinoma cells via inducing miR\u2010195"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8156-5980","authenticated-orcid":false,"given":"Tian","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Oncology RenMin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9133-6500","authenticated-orcid":false,"given":"Guang","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Oncology RenMin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Oncology RenMin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,13]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8245345"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599813512783"},{"issue":"4","key":"e_1_2_6_1_4_1","first-page":"1658","article-title":"miR\u2010195 inhibits cell proliferation and angiogenesis in human prostate cancer by downregulating PRR11 expression","volume":"39","author":"Cai C.","year":"2018","journal-title":"Oncology Reports"},{"issue":"7","key":"e_1_2_6_1_5_1","first-page":"1463","article-title":"Long non\u2010coding RNA DGCR5 is involved in the regulation of proliferation, migration and invasion of lung cancer by targeting miR\u20101180","volume":"7","author":"Chen E. 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