{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:11:53Z","timestamp":1792289513778,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,12,10]],"date-time":"2018-12-10T00:00:00Z","timestamp":1544400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,12,10]],"date-time":"2018-12-10T00:00:00Z","timestamp":1544400000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    \u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010Mannuronic acid (M2000), a novel non\u2010steroidal anti\u2010inflammatory drug (NSAID) with immunosuppressive properties, has been previously shown to exhibit potential therapeutic effects on autoimmune diseases. Immunosuppression therapy has been a standard approach for myelodysplastic syndrome (MDS) for many years. We evaluated the effect of M2000 on isolated peripheral blood mononuclear cells (PBMCs) from patients with MDS. The PBMCs were isolated from 13 patients with MDS and 13 normal donors. The cells were then treated with low, moderate, and high doses of M2000 and diclofenac as a control group. The level of interleukin (IL)\u20106, tumor necrosis factor alpha (TNF\u2010\u03b1), IL\u20103, granulocyte colony\u2010stimulating factor (G\u2010CSF) gene expression and the serum level of IL\u20106 and TNF\u2010\u03b1 production were evaluated by real\u2010time polymerase chain reaction and enzyme\u2010linked immunosorbent assay methods, respectively. Our findings indicated a significant reduction in the production of IL\u20106 and TNF\u2010\u03b1 as inflammatory cytokines. Furthermore, the level of G\u2010CSF gene expression was significantly increased. In conclusion, M2000, a newly designed NSAID, has a remarkable effect on isolated PBMC in patients with MDS, which might bring a potential hope for its oral administrations in these patients.\n                  <\/jats:p>","DOI":"10.1002\/jcp.27966","type":"journal-article","created":{"date-parts":[[2018,12,10]],"date-time":"2018-12-10T23:15:16Z","timestamp":1544483716000},"page":"12971-12977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["An in vitro assessment for evaluating the efficiency of \u03b2\u2010\n                    <scp>d<\/scp>\n                    \u2010mannuronic acid (M2000) in myelodysplastic syndrome"],"prefix":"10.1002","volume":"234","author":[{"given":"Tahereh","family":"Bakhtiari","sequence":"first","affiliation":[{"name":"Department of Immunology School of Public Health, Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afshin","family":"Ghaderi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Hematology and Medical Oncology Ward, Cancer Research Centre. Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Science Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayyed Reza","family":"Safaee Nodehi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Hematology and Medical Oncology Ward, Cancer Research Centre. Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Science Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Aghazadeh","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Public Health, Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzaneh","family":"Tofighi Zavareh","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Public Health, Tehran University of Medical Sciences Tehran Iran"},{"name":"Research Centre for Immunodeficiencies, Children's Medical Centre, Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahimeh","family":"Jafarnezhad\u2010Ansariha","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Public Health, Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anis","family":"Barati","sequence":"additional","affiliation":[{"name":"Department of Biology College of Sciences, Shiraz University Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1996-2747","authenticated-orcid":false,"given":"Abbas","family":"Mirshafiey","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Public Health, Tehran University of Medical Sciences Tehran Iran"},{"name":"Research Centre for Immunodeficiencies, Children's Medical Centre, Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,10]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570163814666170321113059"},{"issue":"5","key":"e_1_2_6_1_3_1","first-page":"433","article-title":"Hematological improvement of patients with active rheumatoid arthritis by \u03b2\u2010d\u2010mannuronic acid (M2000) as a novel NSAID with immunosuppressive property","volume":"16","author":"Ahmadi H.","year":"2017","journal-title":"Iranian Journal of Allergy, Asthma and Immunology"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-017-0410-8"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-018-0475-z"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/sji.12519"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2011.07.003"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-06-653113"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2010.02.003"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2013.09.003"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2015.06.017"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15099"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24984"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2009.09.009"},{"issue":"6","key":"e_1_2_6_1_15_1","first-page":"923","article-title":"Immunoregulatory cytokines gene polymorphisms in Egyptian patients affected with acquired aplastic anemia","volume":"93","author":"El Mahgoub I. 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