{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:41:29Z","timestamp":1793320889034,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,4]],"date-time":"2019-01-04T00:00:00Z","timestamp":1546560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006364","name":"Institut National Du Cancer","doi-asserted-by":"publisher","award":["1 R15 CA173703\u201001"],"award-info":[{"award-number":["1 R15 CA173703\u201001"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cervical cancer is a major public health problem and research using cell culture models has improved understanding of this disease. The human cervix contains three anatomic regions; ectocervix with stratified squamous epithelium, endocervix with secretory epithelium, and transformation zone (TZ) with metaplastic cells. Most cervical cancers originate within the TZ. However, little is known about the biology of TZ cells or why they are highly susceptible to carcinogenesis. The goal of this study was to develop and optimize methods to compare growth and differentiation of cells cultured from ectocervix, TZ or endocervix. We examined the effects of different serum\u2010free media on cell attachment, cell growth and differentiation, and cell population doublings in monolayer culture. We also optimized conditions for organotypic culture of cervical epithelial cells using collagen rafts with human cervical stromal cells. Finally, we present a step\u2010by\u2010step protocol for culturing cells from each region of human cervix.<\/jats:p>","DOI":"10.1002\/jcp.28049","type":"journal-article","created":{"date-parts":[[2019,1,4]],"date-time":"2019-01-04T19:22:44Z","timestamp":1546629764000},"page":"7683-7694","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures"],"prefix":"10.1002","volume":"234","author":[{"given":"Han","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Biology Clarkson University Potsdam New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumona","family":"Mondal","sequence":"additional","affiliation":[{"name":"Department of Mathematics Clarkson University Potsdam New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantanu","family":"Sur","sequence":"additional","affiliation":[{"name":"Department of Biology Clarkson University Potsdam New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0467-8460","authenticated-orcid":false,"given":"Craig D.","family":"Woodworth","sequence":"additional","affiliation":[{"name":"Department of Biology Clarkson University Potsdam New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,4]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.5897\/ijbmbr2013.0154"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006846"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523\u20101747.ep12540422"},{"issue":"1","key":"e_1_2_8_1_5_1","first-page":"117","article-title":"Site and origin of squamous cervical cancer: A histomorphologic study","volume":"62","author":"Burghardt E.","year":"1983","journal-title":"Obstetrics and Gynecology"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI42297"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672\u2010012\u20100125\u20107"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201033865\u20102"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0199761"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25978"},{"issue":"22","key":"e_1_2_8_1_11_1","first-page":"7815","article-title":"Keratinocyte growth conditions modulate telomerase expression, senescence, and immortalization by human papillomavirus type 16 E6 and E7 oncogenes","volume":"63","author":"Fu B.","year":"2003","journal-title":"Cancer Research"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u20106143.2002.20908.x"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(80)90406\u20107"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1202684109"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1267\/ahc.29.51"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.456"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003384"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349\u20107006.1999.tb00828.x"},{"key":"e_1_2_8_1_19_1","volume-title":"Applied linear statistical models","author":"Kutner M. 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