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Aberrant osteoclast function is the main cause of OP, therefore, regulating the differentiation and function of osteoclast is one of the treatment strategies for OP. Pectolinarigenin (PEC) is a medicinal implant isolated from\n                    <jats:italic>Fragrant Eupatorium<\/jats:italic>\n                    . Our experimental data showed that PEC was able to inhibit receptor activator of nuclear factor\u2010\u03baB ligand (RANKL)\u2010induced osteoclastogenesis in vitro, by tartrate\u2010resistant acid phosphatase (TRAcP) staining, Fibrous actin\u00a0ring formation, and hydroxyapatite resorption assays. In terms of mechanism, PEC inhibited the expression of the osteoclastogenesis\u2010related gene, including cathepsin K (\n                    <jats:italic>Ctsk<\/jats:italic>\n                    ), matrix metalloproteinase 9 (\n                    <jats:italic>Mmp9)<\/jats:italic>\n                    , and TRAcP (\n                    <jats:italic>Acp5<\/jats:italic>\n                    ). Western blot analysis demonstrated that PEC could significantly block the activation of RANKL\u2010induced mitogen\u2010activated protein kinase signaling cascades and was able to suppress the protein expression of nuclear factor of activated T\u2010cells and c\u2010Fos. Meanwhile, the intracellular reactive oxygen species levels were also reduced by PEC in a concentration\u2010dependent manner. Further, PEC could prevent the ovariectomy\u2010induced bone loss in vivo. Summarizing all, our data suggested that PEC inhibits osteoclast formation and function and RANKL signaling pathways, and thus could potentially be used in the treatment the osteoclast\u2010related bone loss diseases.\n                  <\/jats:p>","DOI":"10.1002\/jcp.28079","type":"journal-article","created":{"date-parts":[[2019,1,11]],"date-time":"2019-01-11T11:04:05Z","timestamp":1547204645000},"page":"13959-13968","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Pectolinarigenin prevents bone loss in ovariectomized mice and inhibits RANKL\u2010induced osteoclastogenesis via blocking activation of MAPK and NFATc1 signaling"],"prefix":"10.1002","volume":"234","author":[{"given":"Yu","family":"Xiao","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University Nanning Guangxi China"},{"name":"Department of Trauma Orthopedic and Hand Surgery Research Centre 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