{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:25:40Z","timestamp":1787127940052,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T00:00:00Z","timestamp":1546992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T00:00:00Z","timestamp":1546992000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 HL056470"],"award-info":[{"award-number":["R01 HL056470"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Airway smooth muscle (ASM) regulation of airway structure and contractility is critical in fetal\/neonatal physiology in health and disease. Fetal lungs experience higher Ca\n                    <jats:sup>2+<\/jats:sup>\n                    environment that may impact extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    ) sensing receptor (CaSR). Well\u2010known in the parathyroid gland, CaSR is also expressed in late embryonic lung mesenchyme. Using cells from 18\u201022 week human fetal lungs, we tested the hypothesis that CaSR regulates intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    ) in fetal ASM (fASM). Compared with adult ASM, CaSR expression was higher in fASM, while fluorescence Ca\n                    <jats:sup>2+<\/jats:sup>\n                    imaging showed that [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    was more sensitive to altered [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    . The fASM [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    responses to histamine were also more sensitive to [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    (0\u20132\u2009mM) compared with an adult, enhanced by calcimimetic R568 but blunted by calcilytic NPS2143. [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    was enhanced by endogenous CaSR agonist spermine (again higher sensitivity compared with adult). Inhibition of phospholipase C (U73122; siRNA) or inositol 1,4,5\u2010triphosphate receptor (Xestospongin C) blunted [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    sensitivity and R568 effects. NPS2143 potentiated U73122 effects. Store\u2010operated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    entry was potentiated by R568. Traction force microscopy showed responsiveness of fASM cellular contractility to [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    and NPS2143. Separately, fASM proliferation showed sensitivity to [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    and NPS2143. These results demonstrate functional CaSR in developing ASM that modulates airway contractility and proliferation.\n                  <\/jats:p>","DOI":"10.1002\/jcp.28115","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T11:13:45Z","timestamp":1547032425000},"page":"14187-14197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Calcium sensing receptor in developing human airway smooth muscle"],"prefix":"10.1002","volume":"234","author":[{"given":"Anne M.","family":"Roesler","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A.","family":"Wicher","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovanka","family":"Ravix","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Rodney D.","family":"Britt","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Logan","family":"Manlove","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob J.","family":"Teske","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Mayo Clinic Rochester 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