{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:05:59Z","timestamp":1784293559979,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601900"],"award-info":[{"award-number":["81601900"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Clinical studies have indicated that increased serum cholesterol levels raised the risk of tendinopathy in hypercholesterolemia, but the effect of cholesterol on tendon\u2010derived stem cells (TDSCs) and its underlying mechanism have not been studied. The purpose of this study is to investigate the association between cholesterol and tendinopathy in vitro and in vivo, and its underlying molecular mechanism as well. In TDSCs, the effect of cholesterol was assessed by quantitative polymerase chain reaction, western blot analysis, and immunofluorescence staining. Intracellular levels of reactive oxygen species (ROS) was detected, using flow cytometry. The link between nuclear factor (NF)\u2010\u03baB signaling and the effect of cholesterol was evaluated using a representative I\u03baB kinase (IKK) inhibitor, BAY 11\u20107082. In addition, Achilles tendons from apolipoprotein E mice fed with a high\u2010fat diet were histologically assessed using hematoxylin and eosin staining and immunohistochemistry. We found that high cholesterol apparently lowered the expression of tendon cell markers (collagen 1, scleraxis, tenomodulin), and elevated ROS levels via the NF\u2010\u03baB pathway both in vitro and in vivo. The ROS scavenger\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylcysteine (NAC) and BAY 11\u20107082 reversed the inhibiting effect of cholesterol on the tendon\u2010related gene expressions of TDSCs. Moreover, NAC blocked cholesterol\u2010induced phosphorylation of I\u03baB\u03b1 and p65. Significant histological alternation in vivo was shown in Achilles tendon in the hypercholesterolemic group. These results indicated that high cholesterol may inhibit the tendon\u2010related gene expressions in TDSCs via ROS\u2010activated NF\u2010\u043aB signaling, implying pathogenesis of tendinopathy in hypercholesterolemia and suggesting a new mechanism underlying hypercholesterolemia\u2010induced tendinopathy.\n                  <\/jats:p>","DOI":"10.1002\/jcp.28433","type":"journal-article","created":{"date-parts":[[2019,3,2]],"date-time":"2019-03-02T00:46:55Z","timestamp":1551487615000},"page":"18017-18028","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["High cholesterol inhibits tendon\u2010related gene expressions in tendon\u2010derived stem cells through reactive oxygen species\u2010activated nuclear factor\u2010\u03baB signaling"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3322-4345","authenticated-orcid":false,"given":"Kaiqun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopaedics and Traumatology Nanfang Hospital, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganming","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology Nanfang Hospital, Southern Medical University Guangzhou China"},{"name":"Department of Bone and Joint Surgery Baoan District People's Hospital of Shenzhen Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology Nanfang Hospital, Southern Medical University Guangzhou 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Orthopaedics and Traumatology Nanfang Hospital, Southern Medical University Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology Nanfang Hospital, Southern Medical University Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kairui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology Nanfang Hospital, Southern Medical University Guangzhou 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