{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:40:27Z","timestamp":1790322027267,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100011102","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["FP7\/2007\u20102013\/"],"award-info":[{"award-number":["FP7\/2007\u20102013\/"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000783","name":"Research Executive Agency","doi-asserted-by":"publisher","award":["629434"],"award-info":[{"award-number":["629434"]}],"id":[{"id":"10.13039\/501100000783","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Endogenous progenitor cells may participate in cardiac repair after a myocardial infarction (MI). The beta 2 adrenergic receptor (\u00df2\u2010AR) pathway induces proliferation of c\u2010kit+ cardiac progenitor cells (CPC) in vitro. We investigated if \u00df2\u2010AR pharmacological stimulation could ameliorate endogenous CPC\u2010mediated regeneration after a MI. C\u2010kit+ CPC \u00df1\u2010AR and \u00df2\u2010AR expression was evaluated in vivo and in vitro. A significant increase in the percentage of CPCs expressing \u00df1\u2010AR and \u00df2\u2010AR was measured 7 days post\u2010MI. Accordingly, 24\u00a0hrs of low serum and hypoxia in vitro significantly increased CPC \u00df2\u2010AR expression. Cell viability and differentiation assays validated a functional role of CPC \u00df2\u2010AR. The effect of pharmacological activation of \u00df2\u2010AR was studied in C57 mice using fenoterol administered in the drinking water 1 week before MI or sham surgery or at the time of the surgery. MI induced a significant increase in the percentage of c\u2010kit+ progenitor cells at 7 days, whereas pretreatment with fenoterol prolonged this response resulting in a significant elevated number of CPC up to 21 days post\u2010MI. This increased number of CPC correlated with a decrease in infarct size. The immunofluorescence analysis of the heart tissue for proliferation, apoptosis, macrophage infiltration, cardiomyocytes surface area, and vessel density showed significant changes on the basis of surgery but no benefit due to fenoterol treatment. Cardiac function was not ameliorated by fenoterol administration when evaluated by echocardiography. Our results suggest that \u00df2\u2010AR stimulation may improve the cardiac repair process by supporting an endogenous progenitor cell response but is not sufficient to improve the cardiac function.<\/jats:p>","DOI":"10.1002\/jcp.28461","type":"journal-article","created":{"date-parts":[[2019,3,26]],"date-time":"2019-03-26T00:23:22Z","timestamp":1553559802000},"page":"18283-18296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prolonged elevated levels of c\u2010kit+ progenitor cells after a myocardial infarction by beta 2 adrenergic receptor priming"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7773-3024","authenticated-orcid":false,"given":"Amanda","family":"Finan","sequence":"first","affiliation":[{"name":"Physiology &amp; Experimental Medicine of the Heart and Muscles (PhyMedExp), INSERM U1046, CNRS UMR 9214, University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Demion","sequence":"additional","affiliation":[{"name":"Physiology &amp; Experimental Medicine of the Heart and Muscles (PhyMedExp), INSERM U1046, CNRS UMR 9214, University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Sicard","sequence":"additional","affiliation":[{"name":"Physiology &amp; Experimental Medicine of the Heart and Muscles (PhyMedExp), INSERM U1046, CNRS UMR 9214, University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgane","family":"Guisiano","sequence":"additional","affiliation":[{"name":"Physiology &amp; Experimental Medicine of the Heart and Muscles (PhyMedExp), INSERM U1046, CNRS UMR 9214, University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Bideaux","sequence":"additional","affiliation":[{"name":"Physiology &amp; Experimental Medicine of the Heart and Muscles (PhyMedExp), INSERM U1046, CNRS UMR 9214, University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Monceaux","sequence":"additional","affiliation":[{"name":"Physiology &amp; Experimental Medicine of the Heart and Muscles (PhyMedExp), INSERM U1046, CNRS UMR 9214, University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Thireau","sequence":"additional","affiliation":[{"name":"Physiology &amp; Experimental Medicine of the Heart and Muscles (PhyMedExp), INSERM U1046, CNRS UMR 9214, University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Richard","sequence":"additional","affiliation":[{"name":"Physiology &amp; Experimental Medicine of the Heart and Muscles (PhyMedExp), INSERM U1046, CNRS UMR 9214, University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,25]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000139844.15045.F9"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.109.157107"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00687-1"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.9.2797"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13309"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00005.2005"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.59.3.297"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.98.13.1329"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.100.22.2210"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115256"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.082453"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M607391200"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.07.039"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2013.07.016"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2016.06.005"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27019"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653249.2012.684380"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2015.00057"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068528"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007\u20100751"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.300152"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.116.022304"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1611023114"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2016.09.924"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.04.039"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(05)67861\u20100"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08899"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.10.041"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2016.03.017"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.280735"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.22.2645"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.114.002065"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddiscovery.2016.52"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000102042.83024.CA"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvv219"},{"key":"e_1_2_8_1_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102011\u201007\u2010367102"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.9957.3"},{"key":"e_1_2_8_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(12)60195\u20100"},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015\u2010009\u20109050\u20108"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehl401"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.310520"},{"key":"e_1_2_8_1_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12598"},{"key":"e_1_2_8_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.01.016"},{"issue":"3","key":"e_1_2_8_1_45_1","first-page":"309","article-title":"Heart and catecholamines","volume":"46","author":"Prichard B. N.","year":"1991","journal-title":"Acta Cardiologica"},{"key":"e_1_2_8_1_46_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012081"},{"key":"e_1_2_8_1_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehq455"},{"key":"e_1_2_8_1_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c140082"},{"key":"e_1_2_8_1_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006951200"},{"key":"e_1_2_8_1_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395\u2010010\u20100088\u20101"},{"issue":"3","key":"e_1_2_8_1_51_1","first-page":"II13","article-title":"Catecholamines in myocardial ischemia. Systemic and cardiac release","volume":"82","author":"Sch\u00f6mig A.","year":"1990","journal-title":"Circulation"},{"key":"e_1_2_8_1_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvx036"},{"key":"e_1_2_8_1_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/4790563"},{"key":"e_1_2_8_1_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01034.2002"},{"key":"e_1_2_8_1_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1832855100"},{"key":"e_1_2_8_1_56_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2002.282.1.H256"},{"key":"e_1_2_8_1_57_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.308687"},{"key":"e_1_2_8_1_58_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.90.3.1225"},{"key":"e_1_2_8_1_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2011.201"},{"key":"e_1_2_8_1_60_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000087595.17277.73"},{"key":"e_1_2_8_1_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476\u20105381.2010.00813.x"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28461","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.28461","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28461","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:44:48Z","timestamp":1784292288000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.28461"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,25]]},"references-count":60,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1002\/jcp.28461"],"URL":"https:\/\/doi.org\/10.1002\/jcp.28461","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,25]]},"assertion":[{"value":"2018-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}