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The canonical Wnt pathway has recently been associated with pancreas development and the onset of type 2 diabetes in rodent and human but the underlying mechanisms are still unclear. The aim of this work was threefold: (a) to screen for Wnt expressed by murine pancreas\/islet cells, (b) to investigate whether the Wnt gene expression profile can be changed in hyperplastic islets from type 2 prediabetic mice (fed a high\u2010fat diet), and (c) to verify whether soluble factors (namely Wnts) released by pancreatic islets affect insulin secretion and proliferation of a beta\u2010cell line in vitro condition. The majority of the Wnt subtypes are expressed by islet cells, such as Wnts 2, 2b, 3, 3a, 4, 5a, 5b, 6, 7a, 7b, 8a, 8b, 9a, 9b, and 11, while in the whole pancreas homogenates were found the same subtypes, except Wnts 3, 6, 7a, and 7b. Among all the Wnts, the Wnts 3a and 5b showed a significantly increased gene expression in hyperplastic islets from prediabetic mice compared with those from control mice. Furthermore, we observed that coculture with hyperplastic or nonhyperplastic islets did not change the secretory function of the mouse insulinoma clone 6 (MIN6) beta cells but induced a significant increase in cell proliferation in this lineage, which was partially blocked by the IWR\u20101 and IWP\u20102 Wnt inhibitors. In conclusion, we demonstrated that murine pancreas\/islet cells can secrete Wnts, and that islet\u2010released Wnts may participate in the regulation of beta\u2010cell mass under normal and prediabetic conditions.<\/jats:p>","DOI":"10.1002\/jcp.28584","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T00:24:12Z","timestamp":1554769452000},"page":"19852-19865","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Islet cells are the source of Wnts that can induce beta\u2010cell proliferation in vitro"],"prefix":"10.1002","volume":"234","author":[{"given":"Daniela A.","family":"Maschio","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Tissue Biology Institute of Biology, University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valqu\u00edria A.","family":"Matheus","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Tissue Biology Institute of Biology, University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3269-9261","authenticated-orcid":false,"given":"Carla B.","family":"Collares\u2010Buzato","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Tissue Biology Institute of Biology, University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,8]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-016-0058-5"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1479164117738442"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-3701-4-12"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20070072"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.09.130"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2005.10.009"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00489.2011"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.137"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.05.012"},{"key":"e_1_2_8_1_12_1","first-page":"pp. 115","volume-title":"Molecular Nutrition and Diabetes","author":"Collares\u2010Buzato C. 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