{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:05:28Z","timestamp":1784293528411,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T00:00:00Z","timestamp":1554249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T00:00:00Z","timestamp":1554249600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The three\u2010dimensional (3D) cell culture model has been increasingly used to study cancer biology and screen for anticancer agents due to its close mimicry to in vivo tumor biopsies. In this study, 3D calcium(Ca)\u2010alginate scaffolds were developed for human glioblastoma cell culture and an investigation of the responses to two anticancer agents, doxorubicin and cordycepin. Compared to the 2D monolayer culture, glioblastoma cells cultured on these 3D Ca\u2010alginate scaffolds showed reduced cell proliferation, increased tumor spheroid formation, enhanced expression of cancer stem cell genes (\n                    <jats:italic>CD133, SOX2, Nestin<\/jats:italic>\n                    , and\n                    <jats:italic>Musashi\u20101<\/jats:italic>\n                    ), and improved expression of differentiation potential\u2010associated genes (\n                    <jats:italic>GFAP<\/jats:italic>\n                    and\n                    <jats:italic>\u03b2\u2010tubulin III<\/jats:italic>\n                    ). Additionally, the vascularization potential of the 3D glioblastoma cells was increased, as indicated by a higher expression of tumor angiogenesis biomarker (\n                    <jats:italic>VEGF<\/jats:italic>\n                    ) than in the cells in 2D culture. To highlight the application of Ca\u2010alginate scaffolds, the 3D glioblastomas were treated with anticancer agents, including doxorubicin and cordycepin. The results demonstrated that the 3D glioblastomas presented a greater resistance to the tested anticancer agents than that of the cells in 2D culture. In summary, the 3D Ca\u2010alginate scaffolds for glioblastoma cells that were developed in this study offer a promising platform for anticancer agent screening and the discovery of drug\u2010resistant mechanisms of cancer.\n                  <\/jats:p>","DOI":"10.1002\/jcp.28608","type":"journal-article","created":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T01:07:41Z","timestamp":1554340061000},"page":"20085-20097","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Fabrication of 3D calcium\u2010alginate scaffolds for human glioblastoma modeling and anticancer drug response evaluation"],"prefix":"10.1002","volume":"234","author":[{"given":"Nipha","family":"Chaicharoenaudomrung","sequence":"first","affiliation":[{"name":"Laboratory of Cell\u2010Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology Suranaree University of Technology Nakhon Ratchasima Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phongsakorn","family":"Kunhorm","sequence":"additional","affiliation":[{"name":"Laboratory of Cell\u2010Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology Suranaree University of Technology Nakhon Ratchasima Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilasinee","family":"Promjantuek","sequence":"additional","affiliation":[{"name":"Laboratory of Cell\u2010Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology Suranaree University of Technology Nakhon Ratchasima Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nudjanad","family":"Heebkaew","sequence":"additional","affiliation":[{"name":"Laboratory of Cell\u2010Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology Suranaree University of Technology Nakhon Ratchasima Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narawadee","family":"Rujanapun","sequence":"additional","affiliation":[{"name":"Laboratory of Cell\u2010Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology Suranaree University of Technology Nakhon Ratchasima Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1474-7792","authenticated-orcid":false,"given":"Parinya","family":"Noisa","sequence":"additional","affiliation":[{"name":"Laboratory of Cell\u2010Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology Suranaree University of Technology Nakhon Ratchasima Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,3]]},"reference":[{"key":"e_1_2_9_1_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-09-0383"},{"key":"e_1_2_9_1_3_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1136\/annrheumdis-2017-eular.4867","article-title":"OP0183 Cordycepin, a novel compound, reduces knee joint pathology and pain in the monosodium iodoacetate (MIA) rat model of osteoarthritis","volume":"76","author":"Ashraf S.","year":"2017","journal-title":"Annals of the Rheumatic Diseases"},{"key":"e_1_2_9_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.10.045"},{"key":"e_1_2_9_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.05.050"},{"key":"e_1_2_9_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12328-8"},{"key":"e_1_2_9_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25781"},{"key":"e_1_2_9_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2005.06.009"},{"key":"e_1_2_9_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2017.10.002"},{"key":"e_1_2_9_1_10_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.11372"},{"key":"e_1_2_9_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.10.056"},{"key":"e_1_2_9_1_12_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S71957"},{"key":"e_1_2_9_1_13_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/8\/4\/045005"},{"key":"e_1_2_9_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.04.012"},{"key":"e_1_2_9_1_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6TB01713D"},{"key":"e_1_2_9_1_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053708"},{"key":"e_1_2_9_1_17_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e318212a89f"},{"issue":"1","key":"e_1_2_9_1_18_1","first-page":"250","article-title":"Three\u2010dimensional hydrogel is suitable for targeted investigation of amoeboid migration of glioma cells","volume":"17","author":"Huang Y.","year":"2018","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_9_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.03.026"},{"key":"e_1_2_9_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2009.12.001"},{"key":"e_1_2_9_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.07.028"},{"key":"e_1_2_9_1_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/15583724.2015.1040552"},{"key":"e_1_2_9_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-011-0749-4"},{"key":"e_1_2_9_1_24_1","doi-asserted-by":"crossref","unstructured":"Kapa\u0142czy\u0144ska M. Kolenda T. Przyby\u0142a W. Zaj\u0105czkowska M. Teresiak A. Filas V. \u2026Lamperska K.(2016).2D and 3D cell cultures\u2013a comparison of different types of cancer cell cultures","DOI":"10.5114\/aoms.2016.63743"},{"key":"e_1_2_9_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.03.062"},{"key":"e_1_2_9_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2015.06.059"},{"key":"e_1_2_9_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22681-3"},{"key":"e_1_2_9_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00201-5"},{"key":"e_1_2_9_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800249-0.00009-3"},{"issue":"3","key":"e_1_2_9_1_30_1","first-page":"1119","article-title":"Tumour\u2013endothelial cell communications: Important and indispensable mediators of tumour angiogenesis","volume":"36","author":"Lopes\u2010Bastos B. M.","year":"2016","journal-title":"Anticancer Research"},{"key":"e_1_2_9_1_31_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10885"},{"key":"e_1_2_9_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.03.009"},{"key":"e_1_2_9_1_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2236"},{"key":"e_1_2_9_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.10067"},{"key":"e_1_2_9_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/pi.2378"},{"issue":"29","key":"e_1_2_9_1_36_1","first-page":"39","article-title":"Making tissue engineering scaffolds work. Review: The application of solid freeform fabrication technology to the production of tissue engineering scaffolds","volume":"5","author":"Sachlos E.","year":"2003","journal-title":"European Cells & Materials"},{"key":"e_1_2_9_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2015.03.061"},{"key":"e_1_2_9_1_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2017-12.2012"},{"key":"e_1_2_9_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(96)00181-0"},{"key":"e_1_2_9_1_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1951"},{"key":"e_1_2_9_1_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/489036a"},{"key":"e_1_2_9_1_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma6041285"},{"key":"e_1_2_9_1_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/23294515.2015.1034381"},{"key":"e_1_2_9_1_44_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6072"},{"key":"e_1_2_9_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00167-4"},{"key":"e_1_2_9_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2010.08.015"},{"key":"e_1_2_9_1_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-016-0387-y"},{"key":"e_1_2_9_1_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b10673"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28608","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.28608","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28608","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:56:45Z","timestamp":1784293005000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.28608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,3]]},"references-count":47,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1002\/jcp.28608"],"URL":"https:\/\/doi.org\/10.1002\/jcp.28608","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,3]]},"assertion":[{"value":"2019-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}