{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:05:50Z","timestamp":1784293550783,"version":"3.55.0"},"reference-count":91,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T00:00:00Z","timestamp":1554768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T00:00:00Z","timestamp":1554768000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["58538"],"award-info":[{"award-number":["58538"]}],"id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Implantation of embryos needs endometrial receptivity. Mineralocorticoids is one of the causes influencing the implantation window. This study targeted to evaluation fludrocortisone different properties on endometrial receptivity. The objective of this study was to assess whether treatment with fludrocortisone could impact the expression of diverse genes and proteins that are involved in uterine receptivity in mice. In this study, 40 female adult BALB\/c mice were used. The samples were allocated to four groups of ten. Control group (C) received: vehicle; fludrocortisone group (FCA): received 1.5\u2009mg\/kg fludrocortisone; PP242 group (PP242): received 30\u2009mg\/kg PP242; fludrocortisone+PP242 group (FCA+PP242): received fludrocortisone and PP242. Mice were killed on window implantation day after mating and confirmed pregnancy. The endometrial epithelium of mouse was collected to assess mRNA expression of leukemia inhibitory factor (LIF), mucin\u20101 (MUC1), heparin\u2010binding epidermal growth factor (HB\u2010EGF), (Msx.1), miRNA Let\u20107a, and miRNA 223\u20103p as well as protein expression of extracellular signal\u2010regulated kinase 1\/2 (ERK1\/2), mammalian target of rapamycin (mTOR), and eukaryotic translation initiation factor 4E\u2010binding protein 1 (4E\u2010BP1) in the uterine using real\u2010time PCR and western blot, respectively. In comparison with the control group, fludrocortisone administration upregulated the expression of LIF, HB\u2010EGF, Msx.1, miRNA Let\u20107a, ERK1\/2, and mTOR in the epithelial endometrium. The PP242\u2010treated group demonstrated a significant rise in the expression of MUC1, miRNA 223\u20103p and a remarkable decline in ERK1\/2 and p\u20104E\u2010BP1 levels in comparison with the control group. Combination therapy of (FCA+PP242) resulted in a remarkable rise in LIF, Msx\u20101, HB\u2010EGF, ERK1\/2, and mTOR levels, in comparison with the PP242 group. Furthermore, combination therapy of (FCA+PP242) downregulated the expression of MUC1 in comparison with the PP242\u2010treated group. According to the results, fludrocortisone affected uterine receptivity possibly by means of modulating the expression of genes involved in the uterine receptivity and activation of the ERK1\/2\u2010mTOR pathway.<\/jats:p>","DOI":"10.1002\/jcp.28609","type":"journal-article","created":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:18:29Z","timestamp":1554855509000},"page":"20098-20110","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The effect of fludrocortisone on the uterine receptivity partially mediated by ERK1\/2\u2010mTOR pathway"],"prefix":"10.1002","volume":"234","author":[{"given":"Mohammad Bakhtiar","family":"Hesam Shariati","sequence":"first","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas Majdi","family":"Seghinsara","sequence":"additional","affiliation":[{"name":"Department of Anatomical Sciences, Faculty of Medicine Tabriz University of Medical Science Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naser","family":"Shokrzadeh","sequence":"additional","affiliation":[{"name":"Infertility and Reproductive Health research center, Health Research Institute Babol University of Medical Sciences Babol Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8816-2797","authenticated-orcid":false,"given":"Behrooz","family":"Niknafs","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology, Faculty of Advanced Medical Sciences Tabriz University of Medical Science Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,9]]},"reference":[{"key":"e_1_2_10_1_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201500042"},{"key":"e_1_2_10_1_3_1","doi-asserted-by":"publisher","DOI":"10.5653\/cerm.2016.43.1.15"},{"key":"e_1_2_10_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmau.2016.07.004"},{"key":"e_1_2_10_1_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0290153"},{"key":"e_1_2_10_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/8.3.269"},{"key":"e_1_2_10_1_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2000-12565"},{"key":"e_1_2_10_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(00)00200-8"},{"key":"e_1_2_10_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_10_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/2.1.31"},{"key":"e_1_2_10_1_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod55.5.1098"},{"key":"e_1_2_10_1_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.105.2.397"},{"key":"e_1_2_10_1_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-2-4"},{"key":"e_1_2_10_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(99)00306-4"},{"key":"e_1_2_10_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.2514"},{"key":"e_1_2_10_1_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-09-0090"},{"key":"e_1_2_10_1_17_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0176OC"},{"key":"e_1_2_10_1_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/det462"},{"key":"e_1_2_10_1_19_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.1.294"},{"key":"e_1_2_10_1_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2003-0403"},{"key":"e_1_2_10_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(99)00046-7"},{"key":"e_1_2_10_1_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmm011"},{"issue":"2","key":"e_1_2_10_1_23_1","first-page":"1155","article-title":"MicroRNA\u2010223\u20103p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice","volume":"8","author":"Dong X.","year":"2016","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_10_1_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0910359"},{"key":"e_1_2_10_1_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100393"},{"key":"e_1_2_10_1_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI73935"},{"key":"e_1_2_10_1_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1998.0394"},{"key":"e_1_2_10_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2003.10.007"},{"key":"e_1_2_10_1_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402597101"},{"key":"e_1_2_10_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ame2.12002"},{"key":"e_1_2_10_1_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a135762"},{"key":"e_1_2_10_1_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-55972-6_2"},{"key":"e_1_2_10_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20307"},{"issue":"6","key":"e_1_2_10_1_34_1","first-page":"841","article-title":"MiR\u2010223 suppresses endometrial carcinoma cells proliferation by targeting IGF\u20101R","volume":"6","author":"Huang K.","year":"2014","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_10_1_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.304626"},{"key":"e_1_2_10_1_36_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2014-106"},{"key":"e_1_2_10_1_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000172622.51973.f5"},{"key":"e_1_2_10_1_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00021.2002"},{"key":"e_1_2_10_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.21066"},{"issue":"87","key":"e_1_2_10_1_40_1","doi-asserted-by":"crossref","DOI":"10.3791\/51513-v","article-title":"Two methods for establishing primary human endometrial stromal cells from hysterectomy specimens","author":"Jividen K.","year":"2014","journal-title":"Journal of Visualized Experiments: JoVE"},{"key":"e_1_2_10_1_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00577.2004"},{"key":"e_1_2_10_1_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfm386"},{"key":"e_1_2_10_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00809-6"},{"issue":"9","key":"e_1_2_10_1_44_1","first-page":"3355","article-title":"Multiple roles for heparin\u2010binding epidermal growth factor\u2010like growth factor are suggested by its cell\u2010specific expression during the human endometrial cycle and early placentation","volume":"84","author":"Leach R. E.","year":"1999","journal-title":"The Journal of Clinical Endocrinology & Metabolism"},{"key":"e_1_2_10_1_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075282"},{"key":"e_1_2_10_1_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10129"},{"key":"e_1_2_10_1_47_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.3.4991"},{"key":"e_1_2_10_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(02)00806-2"},{"key":"e_1_2_10_1_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037039"},{"key":"e_1_2_10_1_50_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1998.13.2.175"},{"key":"e_1_2_10_1_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01678.x"},{"key":"e_1_2_10_1_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00099"},{"key":"e_1_2_10_1_53_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000118519.66430.22"},{"issue":"3","key":"e_1_2_10_1_54_1","first-page":"809","article-title":"Comparative effects of PP242 and rapamycin on mTOR signalling and NOTCH signalling in leukemia cells","volume":"33","author":"Ono A.","year":"2013","journal-title":"Anticancer Research"},{"key":"e_1_2_10_1_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.10159"},{"key":"e_1_2_10_1_56_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2014-1074"},{"key":"e_1_2_10_1_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_10_1_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22643"},{"key":"e_1_2_10_1_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.370"},{"key":"e_1_2_10_1_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew186"},{"key":"e_1_2_10_1_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2498"},{"key":"e_1_2_10_1_62_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/201514","article-title":"Leukemia inhibitory factor: Roles in embryo implantation and in nonhormonal contraception","volume":"2014","author":"Salleh N.","year":"2014","journal-title":"The Scientific World Journal"},{"key":"e_1_2_10_1_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/374460a0"},{"key":"e_1_2_10_1_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(02)03375-7"},{"key":"e_1_2_10_1_65_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2004-0110"},{"key":"e_1_2_10_1_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2009.09.036"},{"issue":"23","key":"e_1_2_10_1_67_1","first-page":"6220","article-title":"Semi\u2010quantitative analysis of endometrial receptivity marker mRNA expression in the mid\u2010secretory endometrium of patients with uterine fibromas","volume":"11","author":"Shokrzadeh N.","year":"2012","journal-title":"African Journal of Biotechnology"},{"key":"e_1_2_10_1_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27969"},{"key":"e_1_2_10_1_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23061"},{"key":"e_1_2_10_1_70_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.14.8.0498"},{"key":"e_1_2_10_1_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2004.06.057"},{"key":"e_1_2_10_1_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/359076a0"},{"key":"e_1_2_10_1_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12248"},{"key":"e_1_2_10_1_74_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.8.7628404"},{"key":"e_1_2_10_1_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12513"},{"key":"e_1_2_10_1_76_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00062.2005"},{"key":"e_1_2_10_1_77_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.4.1287"},{"key":"e_1_2_10_1_78_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.130345wh"},{"key":"e_1_2_10_1_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.98"},{"key":"e_1_2_10_1_80_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11523-011-0179-4"},{"key":"e_1_2_10_1_81_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01152.x"},{"key":"e_1_2_10_1_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1808"},{"key":"e_1_2_10_1_83_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610023200"},{"key":"e_1_2_10_1_84_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep07546"},{"key":"e_1_2_10_1_85_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-09-310888"},{"key":"e_1_2_10_1_86_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508056112"},{"key":"e_1_2_10_1_87_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0140160"},{"key":"e_1_2_10_1_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(88)90006-4"},{"key":"e_1_2_10_1_89_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707909104"},{"key":"e_1_2_10_1_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2010.05.002"},{"key":"e_1_2_10_1_91_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-15-0142"},{"key":"e_1_2_10_1_92_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e14-05-0998"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28609","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.28609","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28609","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:56:48Z","timestamp":1784293008000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.28609"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,9]]},"references-count":91,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1002\/jcp.28609"],"URL":"https:\/\/doi.org\/10.1002\/jcp.28609","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,9]]},"assertion":[{"value":"2019-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}