{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:05:42Z","timestamp":1784293542911,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T00:00:00Z","timestamp":1554422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T00:00:00Z","timestamp":1554422400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Science and Technology Committee of Tongzhou District, Beijing City","award":["KJ2016CX009-08"],"award-info":[{"award-number":["KJ2016CX009-08"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study aims to determine the feasibility of using oligodeoxynucleotides with unmethylated cytosine\u2010guanine dinucleotide sequences (CpG ODN) as an immunity protection strategy for a mouse model of acute respiratory distress syndrome (ARDS). This is a prospective laboratory animal investigation. Twenty\u2010week\u2010old BALB\/c mice in Animal research laboratory were randomized into groups. An ARDS model was induced in mice using lipopolysaccharides (LPSs). CpG ODN was intranasally and transrectally immunized before or after the 3rd and 7th days of establishing the ARDS model. Mice were euthanized on Day 7 after the second immunization. Then, retroorbital bleeding was carried out and the chest was rapidly opened to collect the trachea and tissues from both lungs for testing. CpG ODN significantly improved the pathologic impairment in mice lung, especially after the intranasal administration of 50\u2009\u03bcg. This resulted in the least severe lung tissue injury. Furthermore, interleukin\u20106 (IL\u20106) and IL\u20108 concentrations were lower, which was second to mice treated with the rectal administration of 20 \u00b5g CpG ODN. In contrast, the nasal and rectal administration of CpG ODN in BALB\/c mice before LPS immunization did not appear to exhibit any significant protective effects. The intranasal administration of CpG ODN may be\u00a0a potential treatment approach to ARDS. More studies are needed to further determine the protective mechanism of CpG ODN.<\/jats:p>","DOI":"10.1002\/jcp.28613","type":"journal-article","created":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T07:13:00Z","timestamp":1554534780000},"page":"20118-20127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A study on the protective effects of CpG oligodeoxynucleotide\u2010induced mucosal immunity against lung injury in a mouse acute respiratory distress syndrome model"],"prefix":"10.1002","volume":"234","author":[{"given":"Guan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Critical Care Medicine Beijing Tiantan Hospital, Capital Medical University Beijing China"},{"name":"Department of Critical Care Medicine Beijing Luhe Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zong\u2010Jian","family":"Liu","sequence":"additional","affiliation":[{"name":"Center Laboratory Beijing Luhe Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Center Laboratory Beijing Luhe Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng\u2010Ge","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine Beijing Luhe Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine Beijing Luhe Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Bing","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine Beijing Luhe Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9696-2324","authenticated-orcid":false,"given":"Jian\u2010Xin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine Beijing Tiantan Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,5]]},"reference":[{"key":"e_1_2_10_1_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623315598399"},{"key":"e_1_2_10_1_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199605303342201"},{"key":"e_1_2_10_1_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2012.5669"},{"key":"e_1_2_10_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(67)90168-7"},{"key":"e_1_2_10_1_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0291"},{"key":"e_1_2_10_1_7_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2014.905536"},{"key":"e_1_2_10_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2016.11.001"},{"key":"e_1_2_10_1_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199801000-00011"},{"key":"e_1_2_10_1_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mop.0000147907.30720.04"},{"key":"e_1_2_10_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)63467-8"},{"key":"e_1_2_10_1_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402513"},{"key":"e_1_2_10_1_13_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.49.4010"},{"key":"e_1_2_10_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.2.702-707.2002"},{"key":"e_1_2_10_1_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000001005"},{"key":"e_1_2_10_1_16_1","first-page":"1","volume-title":"Effect and possible mechanism of CpG ODN on lung fibrosis","author":"Li L.","year":"2010"},{"key":"e_1_2_10_1_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-016-0068-5"},{"key":"e_1_2_10_1_18_1","first-page":"1","article-title":"Effect of CpG\u2010ODN1826 to radiation\u2010induced lung injury in mice","author":"Li X.","year":"2014","journal-title":"Biological Research"},{"key":"e_1_2_10_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(01)00396-3"},{"key":"e_1_2_10_1_20_1","first-page":"287","article-title":"Immunization update, 2002","volume":"85","author":"Maxim R. B.","year":"2002","journal-title":"Medicine and Health, Rhode Island"},{"key":"e_1_2_10_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20360"},{"key":"e_1_2_10_1_22_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201012-2090OC"},{"key":"e_1_2_10_1_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1401520"},{"key":"e_1_2_10_1_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31822d7bf0"},{"key":"e_1_2_10_1_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.39537.939039.BE"},{"key":"e_1_2_10_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2011.11.004"},{"key":"e_1_2_10_1_27_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2011.1435"},{"key":"e_1_2_10_1_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050333"},{"key":"e_1_2_10_1_29_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.69.2.253"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.28613","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28613","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.28613","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28613","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:57:15Z","timestamp":1784293035000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.28613"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,5]]},"references-count":28,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1002\/jcp.28613"],"URL":"https:\/\/doi.org\/10.1002\/jcp.28613","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,5]]},"assertion":[{"value":"2018-12-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}