{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T09:59:02Z","timestamp":1790071142275,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T00:00:00Z","timestamp":1555113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T00:00:00Z","timestamp":1555113600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Carboxyl\u2010terminus of Hsc70 interacting protein (CHIP) is a chaperone\u2010dependent E3\u2010ubiquitin ligase with important function in protein quality control system. In the current research endeavor, we have investigated the putative role of CHIP in lipopolysaccharides (LPS)\u2010induced cardiomyopathies. Basically, H9c2 cardiomyoblasts were transfected with CHIP for 24 hr, and thereafter, treated with LPS for 12 hr. Concomitantly, western blot analysis, actin staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and coimmunoprecipitation studies were performed to investigate the underlying intricacies. Interestingly, western blot analysis revealed that the expression of hypertrophy and apoptosis\u2010related proteins were considerably reduced following overexpression of CHIP. Moreover, Actin staining and TUNEL assay further ascertained the attenuation of cardiac hypertrophy and apoptosis following overexpression of CHIP respectively. These aspects instigate the role of CHIP in attenuation of LPS\u2010induced cardiomyopathies. Additionally and importantly, co\u2010immunoprecipitation and western blot studies revealed that CHIP plausibly promotes degradation of nuclear factor of activated T cells 3 (NFATc3) through ubiquitin\u2010proteasomal pathway. Taken together, our study reveals that CHIP attenuates LPS\u2010induced cardiac hypertrophy and apoptosis perhaps by promoting NFATc3 proteasomal degradation.<\/jats:p>","DOI":"10.1002\/jcp.28614","type":"journal-article","created":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T04:49:57Z","timestamp":1555130997000},"page":"20128-20138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["CHIP attenuates lipopolysaccharide\u2010induced cardiac hypertrophy and apoptosis by promoting NFATc3 proteasomal degradation"],"prefix":"10.1002","volume":"234","author":[{"given":"Chun\u2010Nun","family":"Chao","sequence":"first","affiliation":[{"name":"Department of Biotechnology Asia University Taichung Taiwan"},{"name":"Department of Pediatrics Ditmanson Medical Foundation Chia\u2010Yi Christian Hospital Chiayi 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