{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:46:33Z","timestamp":1790516793835,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870800"],"award-info":[{"award-number":["81870800"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Science and Technology Department of Sichuan Province","doi-asserted-by":"publisher","award":["No. 2015JY0146"],"award-info":[{"award-number":["No. 2015JY0146"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Satellite glial cells (SGCs) activation in the trigeminal ganglia (TG) is critical in various abnormal orofacial sensation in nerve injury and inflammatory conditions. SGCs express several subtypes of P2 purinergic receptors contributing to the initiation and maintenance of neuropathic pain. The P2Y\n                    <jats:sub>14<\/jats:sub>\n                    receptor, a G\u2010protein\u2010coupled receptor activated by uridine diphosphate (UDP)\u2010glucose and other UDP sugars, mediates various physiologic events such as immune, inflammation, and pain. However, the expression, distribution, and function of P2Y\n                    <jats:sub>14<\/jats:sub>\n                    receptor in SGCs remains largely unexplored. Our study reported the expression and functional identification of P2Y\n                    <jats:sub>14<\/jats:sub>\n                    receptor in SGCs. SGCs were isolated from TG of rat, and the P2Y\n                    <jats:sub>14<\/jats:sub>\n                    receptor expression was examined using immunofluorescence technique. Cell proliferation and viability were examined via cell counting kit\u20108 experiment. Immunofluorescence demonstrated the presence of P2Y\n                    <jats:sub>14<\/jats:sub>\n                    receptor in SGCs. Immunofluorescence and western blot showed that UDP\u2010glucose treatment upregulated glial fibrillary acid protein, a common marker for glial activation. Extracellular UDP\u2010glucose enhanced the phosphorylation of extracellular signal\u2010regulated kinase (ERK), c\u2010Jun N\u2010terminal kinase (JNK), and p38, which were both abolished by the P2Y\n                    <jats:sub>14<\/jats:sub>\n                    receptor inhibitor (PPTN). Furthermore, quantitative reverse transcription polymerase chain reaction and enzyme\u2010linked immunosorbent assay demonstrated that extracellular UDP\u2010glucose significantly enhanced interleukin\u20101\u03b2 (IL\u20101\u03b2) and chemokine CCL2 (CCL2) release, which was abolished by PPTN and significantly decreased by inhibitors of MEK\/ERK (U0126) and p38 (SB202190). Our findings directly proved the functional presence of P2Y\n                    <jats:sub>14<\/jats:sub>\n                    receptor in SGCs. It was also verified that P2Y\n                    <jats:sub>14<\/jats:sub>\n                    receptor activation was involved in activating SGCs, phosphorylating MAPKs, and promoting the secretion of IL\u20101\u03b2 and CCL2 via ERK and p38 pathway.\n                  <\/jats:p>","DOI":"10.1002\/jcp.28726","type":"journal-article","created":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T05:53:06Z","timestamp":1556517186000},"page":"21199-21210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["P2Y\n                    <sub>14<\/sub>\n                    receptor is functionally expressed in satellite glial cells and mediates interleukin\u20101\u03b2 and chemokine CCL2 secretion"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0034-9686","authenticated-orcid":false,"given":"Jiu","family":"Lin","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral 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University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010ke","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010yi","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da\u2010qing","family":"Liao","sequence":"additional","affiliation":[{"name":"Laboratory of Anesthesia &amp; Critical Care Medicine, Translational Neuroscience Center West China Hospital of Sichuan University Chengdu 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