{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:01:57Z","timestamp":1790074917796,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572621"],"award-info":[{"award-number":["81572621"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adrenal pheochromocytoma (PCC) is a very rare tumor that stems from chromaffin cells, which can develop into malignant tumor. During the operation, abundant blood vessels were often observed in PCC than other adrenal tumors, which increases the difficulty and risk of the surgery. Therefore, it is important to investigate the mechanism of PCC angiogenesis. Twelve surgical specimens of PCC from Ruijin Hospital, Shanghai Jiaotong University were grouped into high and low microvessel density (MVD) group. They were also divided into rich blood supply and nonenriched blood supply group, according to computed tomography (CT) manifestation. Comparative proteomic analysis based on liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) and bioinformatics analysis revealed that 206 proteins differentially regulated in the high MVD group compared with low MVD group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Besides, 61 proteins were discovered to be significantly changed when the 12 samples were grouped according to CT manifestation. By intersecting the differentially changed protein from MVD and CT grouping, 25 proteins were filtered out, with pathological function. COX4I2 was verified to be increased gradually with angiogenesis with increasing severity, and PLAT was shown to be decreased with angiogenesis in PCC, by quantitative reverse\u2010transcription polymerase chain reaction and immunohistochemistry. The quantitative proteomics result indicated that the tumor angiogenesis in PCC is associated with hypoxia. COX4I2 and PLAT were highly correlated with blood supply in PCC which contribute to angiogenesis in PCC, which could be used as biomarkers to better indicate tumor angiogenesis, or targets to regress tumor angiogenesis as treatment.<\/jats:p>","DOI":"10.1002\/jcp.28769","type":"journal-article","created":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T08:43:11Z","timestamp":1558341791000},"page":"22057-22070","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["From clinic to mechanism: Proteomics\u2010based assessment of angiogenesis in adrenal pheochromocytoma"],"prefix":"10.1002","volume":"234","author":[{"given":"Fukang","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Urology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7781-3806","authenticated-orcid":false,"given":"Ran","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Urology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenming","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Urology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingwei","family":"Su","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory for Endocrine Tumors, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China"},{"name":"Laboratory for Endocrine and Metabolic Diseases of Institute of Health Science, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Ye","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory for Endocrine Tumors, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China"},{"name":"Laboratory for Endocrine and Metabolic Diseases of Institute of Health Science, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danfeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory for Endocrine Tumors, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China"},{"name":"Laboratory for Endocrine and Metabolic Diseases of Institute of Health Science, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,20]]},"reference":[{"key":"e_1_2_10_1_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1574892810999150827153642"},{"key":"e_1_2_10_1_3_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2512080743"},{"key":"e_1_2_10_1_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000157980.15710.2b"},{"key":"e_1_2_10_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2018.12.004"},{"key":"e_1_2_10_1_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2017\u201000062"},{"key":"e_1_2_10_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2009.01.021"},{"key":"e_1_2_10_1_8_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.13112460"},{"key":"e_1_2_10_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00104\u2010018\u20100741\u2010z"},{"key":"e_1_2_10_1_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0131234"},{"key":"e_1_2_10_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.12.005"},{"key":"e_1_2_10_1_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms15045323"},{"key":"e_1_2_10_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.047"},{"key":"e_1_2_10_1_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102474\u201014\u2010264"},{"issue":"20","key":"e_1_2_10_1_15_1","first-page":"5839","article-title":"Tumor development is retarded in mice lacking the gene for urokinase\u2010type plasminogen activator or its inhibitor, plasminogen activator inhibitor\u20101","volume":"60","author":"Gutierrez L. S.","year":"2000","journal-title":"Cancer Research"},{"key":"e_1_2_10_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20101119(01)00385\u20107"},{"key":"e_1_2_10_1_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi5012892"},{"issue":"226","key":"e_1_2_10_1_18_1","first-page":"191","article-title":"Antiangiogenic therapy of malignant pheochromocytoma and paraganglioma with the view to the recent scientific developments","volume":"38","author":"Kukla U.","year":"2015","journal-title":"Polski Merkuriusz Lekarski"},{"key":"e_1_2_10_1_19_1","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.1.1.37"},{"key":"e_1_2_10_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456\u2010018\u20109613\u2010x"},{"key":"e_1_2_10_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2017.51"},{"key":"e_1_2_10_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2016.02.005"},{"key":"e_1_2_10_1_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.310482"},{"key":"e_1_2_10_1_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/84.24.1875"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.28769","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28769","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.28769","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28769","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T11:12:55Z","timestamp":1736421175000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.28769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,20]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/jcp.28769"],"URL":"https:\/\/doi.org\/10.1002\/jcp.28769","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,20]]},"assertion":[{"value":"2019-01-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}