{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T11:43:43Z","timestamp":1774007023684,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T00:00:00Z","timestamp":1558656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470326"],"award-info":[{"award-number":["81470326"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nucleolin (NCL, C23) is an important nucleocytoplasmic multifunctional protein. Due to its multifaceted profile and high expression in cancer, NCL is considered to be a marker of drug resistance associated with chemotherapy. However, the biochemical mechanisms in which NCL suppresses drug sensitivity in several cancers have yet to be fully elucidated. This study aims to explore the effect of NCL on drug sensitivity and its potential mechanism in CA46 Burkitt's lymphoma (BL) cells. CA46 BL cells were transfected with lentiviruses carrying the NCL gene (CA46\u2010NCL\u2010overexpression, CA46\u2010NCL\u2010OE), or shRNA sequences that target the endogenous NCL gene (CA46\u2010NCL\u2010knockdown, CA46\u2010NCL\u2010KD). Adriamycin (ADM) IC50 levels for CA46\u2010NCL\u2010overexpressed (OE), CA46\u2010NCL\u2010OE control (OEC), CA46\u2010NCL\u2010knockdown (KD), and CA46\u2010NCL\u2010KD control (KDC) cells were 0.68\u2009\u00b1\u20090.06\u2009\u03bcg\/ml, 0.68\u2009\u00b1\u20090.06\u2009\u03bcg\/ml, 0.68\u2009\u00b1\u20090.06\u2009\u03bcg\/ml, and 0.30\u2009\u00b1\u20090.04\u2009\u03bcg\/ml, respectively. Apoptosis rates were significantly increased following NCL KD, whereas the opposite effect was noted in OE cells. A significant reduction of B\u2010cell lymphoma 2 (Bcl\u20102) mRNA and protein levels in KD cells was observed, while OE cells displayed the opposite effect. The stability of Bcl\u20102 mRNA was influenced by NCL levels, the half\u2010life of which was extended after NCL\u2010OE, whereas it was reduced in KD cells. Finally, results of RNA\u2010immunoprecipitation assays indicated that NCL could bind to Bcl\u20102 mRNA in CA46 cells. Taken together, these results suggested that NCL could mediate Bcl\u20102 expression and stability, and thus enhance ADM resistance in CA46 BL cells.<\/jats:p>","DOI":"10.1002\/jcp.28833","type":"journal-article","created":{"date-parts":[[2019,5,25]],"date-time":"2019-05-25T04:43:48Z","timestamp":1558759428000},"page":"22666-22674","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of nucleolin on adriamycin resistance via the regulation of B\u2010cell lymphoma 2 expression in Burkitt's lymphoma cells"],"prefix":"10.1002","volume":"234","author":[{"given":"Xuqiao","family":"Mei","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"},{"name":"Department of Clinical Laboratory The Affiliated Zhangzhou Municipal Hospital, Fujian Medical University Zhangzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanxin","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donghui","family":"Gan","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"},{"name":"Department of Hematology The Affiliated Hospital of Putian University Putian Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanqin","family":"Cao","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengjun","family":"Wu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weijuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenxing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minhui","family":"Lin","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Yang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4438-2544","authenticated-orcid":false,"given":"Jianda","family":"Hu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hematology Fujian Institute of Hematology, Fujian Medical University Union Hospital Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,24]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-510511"},{"issue":"21","key":"e_1_2_8_1_3_1","first-page":"6101","article-title":"An informatics approach identifying markers of chemosensitivity in human cancer cell lines","volume":"60","author":"Amundson S. A.","year":"2000","journal-title":"Cancer Research"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(03)00453-2"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.43.28057"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2010.03.011"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.10.1236"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0167094"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612822666160302103646"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-009-0705-8"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2011.01.010"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.098830"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.215"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.07.025"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5715"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11523-018-0560-7"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-325"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02942-X"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S154608"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2013.04.001"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-014-0292-9"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170160"},{"issue":"1","key":"e_1_2_8_1_23_1","first-page":"16","article-title":"Bcl\u20102 protein expression and p53 gene mutation in chronic lymphocytic leukemia: Correlation with in vitro sensitivity to chlorambucil and purine analogs","volume":"82","author":"Morabito F.","year":"1997","journal-title":"Haematologica"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.104.006080"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-08-043257"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-10-604975"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20120950"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2008.03.016"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-268003"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01070-9"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20170080"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0b013e3182a92cde"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309111200"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2131"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-5723"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.14.1911"},{"key":"e_1_2_8_1_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.387"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110101"},{"key":"e_1_2_8_1_39_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117965"},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009435200"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-12-471094"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054674"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.28833","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28833","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.28833","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28833","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T11:11:53Z","timestamp":1736421113000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.28833"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,24]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/jcp.28833"],"URL":"https:\/\/doi.org\/10.1002\/jcp.28833","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,24]]},"assertion":[{"value":"2019-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}