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However, it is unclear if proteasomal degradation of Bax is involved in the apoptotic process, especially in heart ischemia\u2010reperfusion (I\/R)\u2010induced injury. In the present study, KPC1 expression was heightened in left ventricular cardiomyocytes of patients with coronary heart disease (CHD), in I\/R\u2010myocardium in vivo and in hypoxia and reoxygenation (H\/R)\u2010induced cardiomyocytes in vitro. Overexpression of KPC1 reduced infarction size and cell apoptosis in I\/R rat hearts. Similarly, the forced expression of KPC1 restored mitochondrial membrane potential (MMP) and cytochrome <jats:italic>c<\/jats:italic> release driven by H\/R in H9c2 cells, whereas reducing cell apoptosis, and knockdown of KPC1 by short\u2010hairpin RNA (shRNA) deteriorated cell apoptosis induced by H\/R. Mechanistically, forced expression of KPC1 promoted Bax protein degradation, which was abolished by proteasome inhibitor MG132, suggesting that KPC1 promoted proteasomal degradation of Bax. Furthermore, KPC1 prevented basal and apoptotic stress\u2010induced Bax translocation to mitochondria. Bax can be a novel target for the antiapoptotic effects of KPC1 on I\/R\u2010induced cardiomyocyte apoptosis and render mechanistic penetration into at least a subset of the mitochondrial effects of KPC1.<\/jats:p>","DOI":"10.1002\/jcp.28854","type":"journal-article","created":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T03:46:58Z","timestamp":1559274418000},"page":"22921-22934","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["KPC1 alleviates hypoxia\/reoxygenation\u2010induced apoptosis in rat cardiomyocyte cells though BAX degradation"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7529-2114","authenticated-orcid":false,"given":"Ye","family":"Yuan","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"},{"name":"Institute of Biomedicine and Key Lab of Human Embryonic Stem Cell of Hubei Province Hubei University of Medicine  Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center  Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Basic Medicine Science Hubei University of Medicine  Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"},{"name":"Institute of Biomedicine and Key Lab of Human Embryonic Stem Cell of Hubei Province Hubei University of Medicine  Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing\u2010Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling\u2010Yun","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010mu","family":"Pan","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010wen","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010ye","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi\u2010You","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; 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