{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:06:49Z","timestamp":1780463209647,"version":"3.54.1"},"reference-count":47,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,5,26]],"date-time":"2019-05-26T00:00:00Z","timestamp":1558828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010322","name":"Pakistan Academy of Sciences","doi-asserted-by":"publisher","award":["5\u20109\/PAS\/22 dated 27.7.2016."],"award-info":[{"award-number":["5\u20109\/PAS\/22 dated 27.7.2016."]}],"id":[{"id":"10.13039\/501100010322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010322","name":"Pakistan Academy of Sciences","doi-asserted-by":"publisher","award":["5\u20139\/PAS\/22"],"award-info":[{"award-number":["5\u20139\/PAS\/22"]}],"id":[{"id":"10.13039\/501100010322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In continuation of the investigation of osteogenic potential of solvent fractions of ethanolic extract of <jats:italic>Cissus quadrangularis<\/jats:italic> (CQ), an ancient medicinal plant, most notably known for its bone\u2010healing properties, to isolate and identify antiosteoporotic compounds. In the current study, we report the effect of hexane fraction (CQ\u2010H) and dichloromethane fraction (CQ\u2010D) of CQ on the differentiation and mineralization of mouse preosteoblast cell line MC3T3\u2010E1 (subclone 4). Growth, viability, and proliferation assays revealed that low concentrations (0.1, 1, and 100\u2009ng\/ml) of both solvent fractions were nontoxic, whereas higher concentrations were toxic to the cells. Differentiation and mineralization of MC3T3\u2010E1 with nontoxic concentrations of CQ\u2010D and CQ\u2010H revealed that CQ\u2010D delayed the mineralization of MC3T3\u2010E1 cells. However, early and enhanced mineralization was observed in cultures treated with nontoxic concentrations of CQ\u2010H, as indicated by Von Kossa staining and expression profile of osteoblast marker genes such as osterix, <jats:italic>Runx2<\/jats:italic>, alkaline phosphatase (<jats:italic>ALP<\/jats:italic>), collagen (<jats:italic>Col1a1<\/jats:italic>), integrin\u2010related bone sialoprotein (<jats:italic>IBSP<\/jats:italic>), osteopontin (<jats:italic>OPN<\/jats:italic>), and osteocalcin (<jats:italic>OCN<\/jats:italic>). These findings suggest CQ\u2010H as the most efficacious solvent fraction for further investigation to isolate and identify the active compounds in CQ\u2010H.<\/jats:p>","DOI":"10.1002\/jcp.28869","type":"journal-article","created":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T04:02:05Z","timestamp":1558929725000},"page":"23082-23096","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Osteogenic potential of hexane and dichloromethane fraction of <i>Cissus quadrangularis<\/i> on murine preosteoblast cell line MC3T3\u2010E1 (subclone 4)"],"prefix":"10.1002","volume":"234","author":[{"given":"Rabail H.","family":"Toor","sequence":"first","affiliation":[{"name":"School of Biological Sciences, Quaid\u2010i\u2010Azam Campus University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabana","family":"Malik","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Quaid\u2010i\u2010Azam Campus University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haleema","family":"Qamar","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Quaid\u2010i\u2010Azam Campus University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faiza","family":"Batool","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Quaid\u2010i\u2010Azam Campus University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maira","family":"Tariq","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Quaid\u2010i\u2010Azam Campus University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zainab","family":"Nasir","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Quaid\u2010i\u2010Azam Campus University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raazia","family":"Tassaduq","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Kinnaird College for Women Lahore Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane B.","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Vermont College of Medicine Burlington Vermont"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet L.","family":"Stein","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Vermont College of Medicine Burlington Vermont"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary S.","family":"Stein","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Vermont College of Medicine Burlington Vermont"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul R.","family":"Shakoori","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Quaid\u2010i\u2010Azam Campus University of the Punjab Lahore Pakistan"},{"name":"Department of Biochemistry, Faculty of Life Sciences University of Central Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,26]]},"reference":[{"issue":"2","key":"e_1_2_8_1_2_1","article-title":"Spectrophotometric and chromatographic analysis of Cissus quadrangularis from Vidisha region","volume":"7","author":"Agrawal N.","year":"2018","journal-title":"International Journal of Scientific Research"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants6040042"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/101206"},{"key":"e_1_2_8_1_5_1","first-page":"16","article-title":"The role of traditional system of medicine in home remedies","volume":"9","author":"Baquar S. R.","year":"2006","journal-title":"Journal of Baqai Medical University"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.03.044"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/0253\u20107613.96310"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974\u20108520.175542"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.4324\/9780203427835"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1263\/jbb.105.586"},{"key":"e_1_2_8_1_11_1","first-page":"44","article-title":"Effect of Cissus quadrangularis in accelerating healing process of experimentally fractured radius\u2010ulna of dog, a preliminary study","volume":"26","author":"Deka D.","year":"1994","journal-title":"Indian Journal of Pharmacology"},{"key":"e_1_2_8_1_12_1","volume-title":"Human Osteoblast Differentiation and Bone Formation: Growth Factors, Hormones and Regulatory Networks (Doctorate)","author":"Eijken M.","year":"2007"},{"issue":"2","key":"e_1_2_8_1_13_1","first-page":"139","article-title":"Analysis of chemical composition of Cissus quadrangularis linn by GC\u2010MS","volume":"5","author":"Eswaran R.","year":"2012","journal-title":"Asian Journal of Pharmaceutical and Clinical Research"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.02.002"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ton.teb.2014.0119"},{"key":"e_1_2_8_1_16_1","unstructured":"IOF (International Osteoporosis Foundation).2019.Facts and Statistics. International Osteoporosis Foundation Website. Available athttp:\/\/www.iofbonehealth.org\/factsstatistics"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/364604"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijem.IJEM_438_17."},{"issue":"2","key":"e_1_2_8_1_19_1","first-page":"140","article-title":"HPLC analysis of extract of in vivo medicinally important climber Cissus quadrangularis L.(HADJOD)","volume":"4","author":"Kalpana T.","year":"2013","journal-title":"International Research Journal of Pharmacy"},{"issue":"1","key":"e_1_2_8_1_20_1","first-page":"35","article-title":"Herbal medicine","volume":"78","author":"Kamboj V. P.","year":"2000","journal-title":"Current science"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2006.08.021"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2010.03.014"},{"key":"e_1_2_8_1_23_1","first-page":"1782","article-title":"Identification of chemical compounds in Cissus quadrangularis L. Variant I of different samples using GC\u2010MS analysis","volume":"4","author":"Kumar S.","year":"2012","journal-title":"Archives of Applied Science Research"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00751"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2011.09.002"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626\u2010008\u20109158\u20101"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.11.094"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.11.026"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.5372\/1905\u20107415.0603.069"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1807\u201059322009001000010"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/03009730902891784"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.5152\/etd.2018.18103"},{"key":"e_1_2_8_1_33_1","first-page":"25","article-title":"Chemical constituents and melanin promotion activity of Cissus quadrangularis Linn","volume":"1","author":"Rao G. V.","year":"2011","journal-title":"Research Journal of Chemical Science"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.75"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973\u20108258.104924"},{"key":"e_1_2_8_1_36_1","first-page":"41","article-title":"Cissus quadrangularis L. phytochemicals, traditional uses and pharmacological activities\u2010a review","volume":"4","author":"Shah U.","year":"2011","journal-title":"International Journal of Pharmacy and Pharmecutical Science"},{"key":"e_1_2_8_1_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035712"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.08.004"},{"key":"e_1_2_8_1_39_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975\u20105950.117884."},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4846"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.5152\/eurjrheum.2016.048"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25449"},{"key":"e_1_2_8_1_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27707"},{"key":"e_1_2_8_1_44_1","first-page":"480","article-title":"Biomechanical and calcium\u201045 studies on the effect of Cissus quadrangularis in fracture repair","volume":"52","author":"Udupa K.","year":"1964","journal-title":"The Indian Journal of Medical Research"},{"key":"e_1_2_8_1_45_1","first-page":"590","article-title":"Cissus quadrangularis in healing of fractures. A clinical study","volume":"38","author":"Udupa K.","year":"1962","journal-title":"Journal of the Indian Medical Association"},{"key":"e_1_2_8_1_46_1","first-page":"26","article-title":"Further studies on the effect of Cissus quadrangularis in accelerating fracture healing","volume":"52","author":"Udupa K.","year":"1964","journal-title":"The Indian Journal of Medical Research"},{"key":"e_1_2_8_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.03.056"},{"issue":"3","key":"e_1_2_8_1_48_1","first-page":"1","article-title":"Anti\u2010osteoporotic effects of medicinal herbs and their mechanisms of action","volume":"1","author":"Zhang Y.","year":"2006","journal-title":"Asian Journal of Traditional Medicines"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.28869","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28869","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.28869","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.28869","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T11:16:17Z","timestamp":1736421377000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.28869"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,26]]},"references-count":47,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/jcp.28869"],"URL":"https:\/\/doi.org\/10.1002\/jcp.28869","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,26]]},"assertion":[{"value":"2019-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}