{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:11:33Z","timestamp":1788293493360,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T00:00:00Z","timestamp":1560124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["132347\/2017\u20102"],"award-info":[{"award-number":["132347\/2017\u20102"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["AUXPE\u2010PNPD 2787\/2010"],"award-info":[{"award-number":["AUXPE\u2010PNPD 2787\/2010"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In asthma, there are high levels of inflammatory mediators, reactive oxygen species (ROS), and eosinophil extracellular traps (EETs) formation in airway. Here, we attempted to investigate the ROS involvement in EETs release and airway inflammation in OVA\u2010challenged mice. Before the intranasal challenge with ovalbumin (OVA), animals were treated with two ROS inhibitors,\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylcysteine (NAC) or diphenyleneiodonium (DPI). We showed that NAC treatment reduced inflammatory cells in lung. DPI and NAC treatments reduced eosinophil peroxidase (EPO), goblet cells hyperplasia, proinflammatory cytokines, NF\u03baB p65 immunocontent, and oxidative stress in lung. However, only the NAC treatment improved mitochondrial energy metabolism. Moreover, the treatments with DPI and NAC reduced EETs release in airway. This is the first study to show that ROS are needed for EETs formation in asthma. Based on our results, NAC and DPI treatments can be an interesting alternative for reducing airway inflammation, mitochondrial damage, and EETs release in asthma.\n                  <\/jats:p>","DOI":"10.1002\/jcp.28931","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T08:20:53Z","timestamp":1560154853000},"page":"23633-23646","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Reactive oxygen species are involved in eosinophil extracellular traps release and in airway inflammation in asthma"],"prefix":"10.1002","volume":"234","author":[{"given":"Josiane Silva","family":"Silveira","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Laboratory of Neuroprotection and Neurometabolic Disease Universidade Federal do Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9ssica Luana","family":"Antunes","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Respirology, Infant Center, Medicine School Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS) Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Benvenutti","family":"Kaiber","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Respirology, Infant Center, Medicine School Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS) Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Severo","family":"da Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Respirology, Infant Center, Medicine School Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS) Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Peil","family":"Marques","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Laboratory of Neuroprotection and Neurometabolic Disease Universidade Federal do Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Silva","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Laboratory of Neuroprotection and Neurometabolic Disease Universidade Federal do Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Benedetti","family":"Gassen","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Science School Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS) Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Vaz","family":"Breda","sequence":"additional","affiliation":[{"name":"Institute of the Brain (INSCER), Medicine School Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS) Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela T. 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