{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:55:20Z","timestamp":1787741720918,"version":"build-2784847793"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T00:00:00Z","timestamp":1561420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["SB\/LS\/YS\u2010356\/2013"],"award-info":[{"award-number":["SB\/LS\/YS\u2010356\/2013"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Adipose tissue is a complex and heterogenic\u00a0tissue exhibiting high variability and appears to have multiple functions, especially in metabolic regulation. Change in carbohydrate source is reported to have a profound effect in the regular functioning of adipocytes. Here, we analyzed the role of two monosaccharides namely, glucose (5.2 and 25\u2009nM), galactose (25\u2009mM), and two disaccharides namely, lactose and sucrose (both at 25\u2009mM) in the adipocyte differentiation process and its utilization by adipocytes as an energy source. The change in cell morphology, adipocyte\u2010specific gene expression, and protein levels were analyzed at three different time points: 2, 6, and 48\u2009hr. Oil Red O staining at Day 8 of differentiation showed that no other carbohydrates were able to increase lipid content as better as 25\u2009mM glucose. Gene expression pattern was altered by the change in glucose concentration and sucrose was able to mimic the effect of glucose even though, the lipid synthesis was solely promoted by high glucose levels. Galactose and lactose did not show any effect in promoting adipocyte differentiation. The expression of PPAR \u03b3 was high in the presence of sucrose and galactose, possibly of adipogenic cocktail in enhancing the expression rather than the effect of carbohydrate. Acarbose, a potent glucosidase inhibitor was able to inhibit the lipid content in adipocytes grown with sucrose as a carbohydrate source and shows the possibility of its direct utilization. Lactate production by cells upon differentiation also proved the possible uptake of glucose after sucrose cleavage.<\/jats:p>","DOI":"10.1002\/jcp.29003","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T23:59:31Z","timestamp":1561507171000},"page":"891-899","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Adipocytes utilize sucrose as an energy source\u2014Effect of different carbohydrates on adipocyte differentiation"],"prefix":"10.1002","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4179-409X","authenticated-orcid":false,"given":"Mahesh S.","family":"Krishna","sequence":"first","affiliation":[{"name":"Division of Cardiovascular Disease and Diabetes Biology Diabetes Biology Lab Rajiv Gandhi Centre for Biotechnology  Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. M.","family":"Revathy","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Disease and Diabetes Biology Diabetes Biology Lab Rajiv Gandhi Centre for Biotechnology  Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Jaleel","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Disease and Diabetes Biology Diabetes Biology Lab Rajiv Gandhi Centre for Biotechnology  Kerala 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