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In this study, we performed a systematic investigation of the tumor microenvironmental and genetic factors associated with melanoma to identify prognostic biomarkers for melanoma. We calculated the immune and stromal scores of melanoma patients from the Cancer Genome Atlas (TCGA) using the ESTIMATE algorithm and found that they were closely associated with patients\u2019 prognosis. Then the differentially expressed genes were obtained based on the immune and stromal scores, and prognostic immune\u2010related genes further identified. Functional analysis and the protein\u2013protein interaction network further revealed that these genes enriched in many immune\u2010related biological processes. In addition, the abundance of six infiltrating immune cells was analyzed using prognostic immune\u2010related genes by TIMER algorithm. The unsupervised clustering analysis using immune\u2010cell proportions revealed eight clusters with distinct survival patterns, suggesting that dendritic cells were most abundant in the microenvironment and CD8<jats:sup>+<\/jats:sup> T cells and neutrophils were significantly related to patients\u2019 prognosis. Finally, we validated these genes in three independent cohorts from the Gene Expression Omnibus database. In conclusion, this study comprehensively analyzed the tumor microenvironment and identified prognostic immune\u2010related biomarkers for melanoma.<\/jats:p>","DOI":"10.1002\/jcp.29018","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T23:59:45Z","timestamp":1561507185000},"page":"1025-1035","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Comprehensive analysis of prognostic immune\u2010related genes in the tumor microenvironment of cutaneous melanoma"],"prefix":"10.1002","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0485-7728","authenticated-orcid":false,"given":"Sheng","family":"Yang","sequence":"first","affiliation":[{"name":"Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University  Nanjing Jiangsu P.R. 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