{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:30:37Z","timestamp":1791246637145,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acute myocardial infarction (AMI) occurred\u00a0in the heart, which underwent\u00a0long\u2010term ischemia, and\u00a0was\u00a0mainly caused by hypoxia. Recently, studies have uncovered the participation of long noncoding RNAs (lncRNAs) in the pathogenesis of heart disease. Here, we planned to probe the role and molecular basis of ANRIL in hypoxia\u2010induced H9c2 cell injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Trypan blue exclusion assay and\u00a0Transwell and flow cytometry assays were conducted to assess hypoxia\u2010induced injury by determining the viability, migration,\u00a0invasion, and apoptosis of H9c2 cells in different conditions, respectively. Gene expressions were evaluated by quantitative real\u2010time polymerase chain reaction or western blot analysis as needed. RNA immunoprecipitation and luciferase reporter assays were applied to confirm the associations among genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ANRIL expression was dramatically enhanced in hypoxia\u2010injured H9c2 cells, and silencing ANRIL aggravated hypoxia\u2010induced H9c2 cell injury. ANRIL positively regulated sirtuin 1 (SIRT1) expression via competitively binding with miR\u20107\u20105p. Moreover, inhibition of miR\u20107\u20105p counteracted ANRIL depletion\u2010exacerbated injury in hypoxic H9c2 cells, meanwhile, forced SIRT1 expression attenuated the injury\u2010promoting effect of miR\u20107\u20105p upregulation on hypoxic H9c2 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings disclosed that ANRIL plays\u00a0a protective\u00a0part in hypoxia\u2010induced H9c2 cell injury via modulating the miR\u20107\u20105p\/SIRT1 axis, suggesting the great potential of ANRIL as a protective target for AMI.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.29031","type":"journal-article","created":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T00:24:02Z","timestamp":1562027042000},"page":"1175-1183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["lncRNA ANRIL protects H9c2 cells against hypoxia\u2010induced injury through targeting the miR\u20107\u20105p\/SIRT1 axis"],"prefix":"10.1002","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9994-8085","authenticated-orcid":false,"given":"Liliang","family":"Shu","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanzhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology The Second Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gongcheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7]]},"reference":[{"key":"e_1_2_8_1_2_1","first-page":"29","article-title":"Long non\u2010coding RNA ANRIL and polycomb in human cancers and cardiovascular disease","volume":"394","author":"Aguilo F.","year":"2015","journal-title":"Current Topics in Microbiology and Immunology"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000267723.65696.4a"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2016.12.029"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3738"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000485"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0722-z"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.14721"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.117.007063"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14011278"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7178"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25576"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000485588"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26304"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000485354"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(74)91367-1"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13790"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.09.040"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.06.122"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3192"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.114.013303"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2014.11.015"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.05.122"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2017.12.018"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-016-0259-5"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-2037"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30677-8"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311802"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.03.010"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12555"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-016-1111-1"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-10547-z"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041523"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520295.2012.730153"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12986"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.303836"},{"key":"e_1_2_8_1_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-010-9368-1"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-016-0349-7"},{"issue":"2","key":"e_1_2_8_1_39_1","first-page":"2422","article-title":"lncRNA TUG1 serves an important role in hypoxia\u2010induced myocardial cell injury by regulating the miR1455pBinp3 axis","volume":"17","author":"Wu Z.","year":"2018","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.5603\/CJ.a2018.0054"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.1159\/000479995"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.07.099"},{"key":"e_1_2_8_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2017.12.097"},{"issue":"5","key":"e_1_2_8_1_44_1","first-page":"3684","article-title":"An immune\u2010related six\u2010lncRNA signature to improve prognosis prediction of glioblastoma multiforme","volume":"55","author":"Zhou M.","year":"2017","journal-title":"Molecular Neurobiology"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.29031","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.29031","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.29031","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T08:12:10Z","timestamp":1757059930000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.29031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/jcp.29031"],"URL":"https:\/\/doi.org\/10.1002\/jcp.29031","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"2019-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}