{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:43:28Z","timestamp":1788615808223,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,7,11]],"date-time":"2019-07-11T00:00:00Z","timestamp":1562803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Medullary thyroid carcinoma (MTC) is a scarce cancerous disease, originating from parafollicular C cells of the thyroid gland. MTC can be manifested as an aggressive carcinoma with metastasis, especially in sporadic forms. Mutations of the rearranged during transfection (<jats:italic>RET<\/jats:italic>) proto\u2010oncogene occurs in all hereditary and a few somatic MTCs, so detection of <jats:italic>RET<\/jats:italic> mutations is needed for prompt and appropriate treatment. MicroRNAs (miRNAs) are noncoding regulatory RNAs. Extensive studies have done in progress or suppression of several types of cancers such as MTCs with the remarkable application as prognostic markers. Of the effective miRNAs in cancers, miR\u2010144 and miR\u201034 were evaluated in our study. Blood samples of 25 <jats:italic>RET<\/jats:italic>\u2010positive and 25 <jats:italic>RET<\/jats:italic>\u2010negative blood samples of patients with MTC were evaluated for these miRNAs, using quantitative real\u2010time polymerase chain reaction (RT\u2010qPCR). Analysis of the results was performed by the 2\n<jats:sup>\u2212\u0394\u0394Ct<\/jats:sup> method, showing that miR\u2010144 and miR\u201034a expression had a relative increase in patients with MTC compared with normal control samples and also in <jats:italic>RET<\/jats:italic> positives versus <jats:italic>RET<\/jats:italic> negatives. We recruited 50 out of 350 MTC plasma samples (27 female and 23 male) which were selected based on <jats:italic>RET<\/jats:italic> mutation in exon 11 (25 <jats:italic>RET<\/jats:italic>\u2010positive and 25 <jats:italic>RET<\/jats:italic>\u2010negative), with a mean\u2009\u00b1\u2009SD age of 37.04\u2009\u00b1\u20091.74 years. Receiver operating characteristic (ROC) curve analysis was done to investigate the prognostic value of these miRNAs; although, they showed no significant prognostic value as MTC biomarkers in plasma samples. In conclusion, miRNAs can be used as biomarkers of cancers such as MTC; however, more studies are needed to find the best candidate miRNAs for the diagnosis of cancers.<\/jats:p>","DOI":"10.1002\/jcp.29055","type":"journal-article","created":{"date-parts":[[2019,7,12]],"date-time":"2019-07-12T00:18:43Z","timestamp":1562890723000},"page":"1366-1373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["An investigation on the expression of miRNAs including miR\u2010144 and miR\u201034a in plasma samples of <i>RET<\/i>\u2010positive and <i>RET<\/i>\u2010negative medullar thyroid carcinoma patients"],"prefix":"10.1002","volume":"235","author":[{"given":"Noushin","family":"Shabani","sequence":"first","affiliation":[{"name":"Department of Biotechnology, School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Sheikholeslami","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Endocrine Research Center (CMERC), Research Institute for Endocrine Sciences Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2592-3528","authenticated-orcid":false,"given":"Mahdi","family":"Paryan","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Production and Research Complex Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Zarif Yeganeh","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Endocrine Research Center (CMERC), Research Institute for Endocrine Sciences Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mohammad","family":"Tavangar","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shariati Hospital Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereidoun","family":"Azizi","sequence":"additional","affiliation":[{"name":"Endocrine Research Center, Research Institute for Endocrine Sciences Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0430-6325","authenticated-orcid":false,"given":"Samira","family":"Mohammadi\u2010Yeganeh","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences  Tehran Iran"},{"name":"Cellular and Molecular Biology Research Center Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Hedayati","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Endocrine Research Center (CMERC), Research Institute for Endocrine Sciences Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,11]]},"reference":[{"key":"e_1_2_10_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-016-9449-0"},{"key":"e_1_2_10_1_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1714"},{"key":"e_1_2_10_1_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1996.03540190047028"},{"key":"e_1_2_10_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2012.09.002"},{"key":"e_1_2_10_1_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135991"},{"key":"e_1_2_10_1_7_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/832163"},{"key":"e_1_2_10_1_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells6020012"},{"issue":"3","key":"e_1_2_10_1_9_1","first-page":"116","article-title":"Genetic mechanisms of neoplasia in MEN 2","volume":"37","author":"Jackson C. 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