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These research are mainly done on the porcine small intestine. However, a report has recently been published about a scaffold produced from the submucosal layer of the ovine small intestine. In the present study, ovine small intestine submucosal (OSIS) was decellularized in a modified manner and its histological, morphological, and biomechanical properties were studied. Decellularization was performed in two phases: physical and chemical. In this method, a chloroform\u2010methanol mixture, enzymatic digestion, and a constant dose of sodium dodecyl sulfate (SDS) was used in the least agitation time and its histological property and biocompatibility were evaluated in the presence of adipose tissue\u2010derived stem cells (ADSCs); furthermore, ADSCs were isolated with a simple method (modified physical washing non\u2010enzymatic isolation). The results were showed that the use of OSIS could be effective and operative. Mechanical properties, histological structure and shape, and glycosaminoglycan content were preserved. In the SDS\u2010treated group, more than 90% of the native cells of tissue were deleted, and also in this group, no toxicity was observed and cell proliferation was supported, compared to the untreated group. Therefore, our results indicate that ADSCs seeded on OSIS scaffold could be used as a new approach in regenerative medicine as hybrid or hydrogel application.<\/jats:p>","DOI":"10.1002\/jcp.29074","type":"journal-article","created":{"date-parts":[[2019,8,10]],"date-time":"2019-08-10T01:58:57Z","timestamp":1565402337000},"page":"1556-1567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Adipose tissue\u2010derived stem cells upon decellularized ovine small intestine submucosa for tissue regeneration: An optimization and comparison method"],"prefix":"10.1002","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8679-3755","authenticated-orcid":false,"given":"Ahad","family":"Ferdowsi Khosroshahi","sequence":"first","affiliation":[{"name":"Department of Anatomical Sciences, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2034-0278","authenticated-orcid":false,"given":"Jafar","family":"Soleimani Rad","sequence":"additional","affiliation":[{"name":"Department of Anatomical Sciences, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7372-5705","authenticated-orcid":false,"given":"Razie","family":"Kheirjou","sequence":"additional","affiliation":[{"name":"Department of Anatomical Sciences, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6426-7375","authenticated-orcid":false,"given":"Babak","family":"Roshangar","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7647-7070","authenticated-orcid":false,"given":"Morteza","family":"Rashtbar","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Research Center Guilan University of Medical Science  Rasht Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9284-0055","authenticated-orcid":false,"given":"Roya","family":"Salehi","sequence":"additional","affiliation":[{"name":"Department of Medical Nanotechnology, Faculty of Advanced Biomedical Sciences Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3487-8834","authenticated-orcid":false,"given":"Mohammad Reza","family":"Ranjkesh","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5329-0951","authenticated-orcid":false,"given":"Leila","family":"Roshangar","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,9]]},"reference":[{"key":"e_1_2_10_1_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/21623945.2017.1312040"},{"key":"e_1_2_10_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.09.013"},{"key":"e_1_2_10_1_4_1","doi-asserted-by":"publisher","DOI":"10.1109\/IEMBS.2005.1617099"},{"key":"e_1_2_10_1_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/2321-3868.118928"},{"key":"e_1_2_10_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.09.061"},{"key":"e_1_2_10_1_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEC.2010.0342"},{"key":"e_1_2_10_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-008-0745-3"},{"issue":"2","key":"e_1_2_10_1_9_1","first-page":"105","article-title":"Treatment of severe burn with DermACELL\u00ae, an acellular dermal matrix","volume":"2","author":"Chen S.\u2010G.","year":"2012","journal-title":"International Journal of Burns and Trauma"},{"key":"e_1_2_10_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.01.057"},{"issue":"1","key":"e_1_2_10_1_11_1","first-page":"195","article-title":"Preparation of lipid extracts from tissues","volume":"2","author":"Christie W. 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