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This ddPCR assay showed high accuracy, sensitivity, and specificity in detecting MCPyV DNA cloned in a recombinant plasmid vector, the control. ddPCR was extended to MCPyV DNA to investigate\/quantify its sequences in clinical samples. Overall, 400 samples were analyzed, that is, 100 chorionic villi and 100 PBMCs, from SA females (<jats:italic>n<\/jats:italic>\u2009=\u2009100), the cases, and 100 chorionic villi and 100 PBMCs from females who underwent voluntary pregnancy interruption (VI, <jats:italic>n<\/jats:italic>\u2009=\u2009100), the control. MCPyV DNA was detected in 4\/100 (4%) and 5\/100 (5%) of SA and VI chorionic villi, respectively. The mean viral DNA load was 1.99 (\u2009\u00b1\u20090.94 standard mean deviation [<jats:italic>SD<\/jats:italic>]) copy\/10<jats:sup>4<\/jats:sup> cells in SA and 3.02 (\u2009\u00b1\u20091.86 [<jats:italic>SD<\/jats:italic>]) copy\/10<jats:sup>4<\/jats:sup> cells in VI. In PBMCs, MCPyV DNA was revealed in 9\/100 (9%) and 14\/100 (14%) of SA and VI, with a mean of 2.09 (\u2009\u00b1\u20091.17 [<jats:italic>SD<\/jats:italic>]) copy\/10<jats:sup>4<\/jats:sup> cells and 4.09 (\u2009\u00b1\u20094.26 [<jats:italic>SD<\/jats:italic>]) copy\/10<jats:sup>4<\/jats:sup> cells in SA and VI, respectively. MCPyV gene expression analysis by quantitative PCR for the large T antigen (LT) and viral capsid protein 1 (VP1) showed their mRNAs in 2\/4 (50%) SA\u2010 and 2\/5 (40%) VI\u2010MCPyV\u2010positive samples. MCPyV DNA was detected\/quantified using the ddPCR technique, in chorionic villi and PBMCs from SA and VI. In our experimental conditions, ddPCR provided a powerful tool to detect\/quantify MCPyV DNA sequences in clinical samples.<\/jats:p>","DOI":"10.1002\/jcp.29213","type":"journal-article","created":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T04:47:55Z","timestamp":1569300475000},"page":"1888-1894","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Droplet\u2010digital PCR assay to detect Merkel cell polyomavirus sequences in chorionic villi from spontaneous abortion affected females"],"prefix":"10.1002","volume":"235","author":[{"given":"Andrea","family":"Tagliapietra","sequence":"first","affiliation":[{"name":"Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology University of Ferrara  Ferrara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John 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