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In this study, we aimed to explore the potential molecular mechanism underlying the therapeutic effect of MSCs\u2010derived exosome reinforced with miR\u201020a in reversing liver I\/R injury. Quantitative real\u2010time polymerase chain reaction, Western blot, and IHC were carried out to compare the differential expressions of miR\u201020a, Beclin\u2010I, FAS, Caspase\u20103, mTOR and P62 in IR rats and normal rats. TUNEL was performed to assess IR\u2010induced apoptosis in IR rats, and luciferase assay was used to confirm the inhibitory effect of miR\u201020a on Beclin\u2010I and FAS expression. Among the 12 candidate microRNAs (miRNAs), miR\u2010486, miR\u201025, miR\u201024, miR\u201020a,miR\u2010466 and miR\u2010433\u20103p were significantly downregulated in I\/R. In particular, miR\u201020a, a miRNA highly expressed in umbilical cord\u2010derived mesenchymal stem cells, was proved to bind to the 3\u02b9 UTR of Beclin\u2010I and FAS to exert an inhibitory effect on their expressions. Since Beclin\u2010I and FAS were aberrantly upregulated in IR, exosomes separated from UC\u2010MSCs showed therapeutic efficacy in reversing I\/R induced apoptosis. In addition, exosomes reinforced with miR\u201020a and separated from UC\u2010MSCs almost fully alleviated I\/R injury. Furthermore, our results showed that miR\u201020a could alleviate the abnormal expression of genes related to apoptosis and autophagy, such as active Caspase\u20103, mTOR, P62, and LC3II. This study presented detailed evidence to clarify the mechanism underlying the therapeutic efficacy of UC\u2010MSCs in the treatment of I\/R injury.<\/jats:p>","DOI":"10.1002\/jcp.29264","type":"journal-article","created":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:57:24Z","timestamp":1569891444000},"page":"3698-3710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["MiR\u201020a\u2010containing exosomes from umbilical cord mesenchymal stem cells alleviates liver ischemia\/reperfusion injury"],"prefix":"10.1002","volume":"235","author":[{"given":"Lin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery Renmin Hospital of Wuhan University Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaolin","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Ezhou Central Hospital Ezhou Hubei 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