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However, the underlying molecular mechanism is not well understood. Herein, we investigated the potential role of RNA binding protein human antigen R (HuR) responsible for the impact of inflammation on pterygium development. The expression of HuR and matrix metallopeptidase\u20109 (MMP\u20109) in pterygium and normal conjunctiva was detected with immunohistochemistry and quantitative reverse transcription polymerase chain reaction (qRT\u2010PCR). The influence of interleukin\u20101\u03b2 (IL\u20101\u03b2) on HuR expression and cellular distribution was determined with western blot and immunofluorescence. The pterygium fibroblast (PTF) migration was determined with scratch wound healing assay and Transwell migration assay. MMP\u20109 production was determined with qRT\u2010PCR and gelatin zymography. The interaction between HuR and MMP\u20109 was investigated with RNP immunoprecipitation (IP) followed by RT\u2010PCR and messenger RNA (mRNA) stability analysis. HuR and MMP\u20109 expression are elevated in pterygium, especially progressive pterygium compared with normal conjunctiva. IL\u20101\u03b2 could increase the expression and nucleus\u2013cytoplasm shuttle of HuR in cultured PTFs. HuR mediated the stimulatory effect of IL\u20101\u03b2 on PTF migration and MMP\u20109 production. HuR bound to MMP\u20109 mRNA and in turn increased it stability. Our results suggest that posttranscriptional regulation of MMP\u20109 via stabilizing mRNA by HuR might contribute to the stimulatory effect of inflammatory factor IL\u20101\u03b2 on pterygium progression. These findings shed light on the pathogenesis of pterygium and provide a promising target for adjuvant treatment of pterygium.<\/jats:p>","DOI":"10.1002\/jcp.29387","type":"journal-article","created":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T11:23:18Z","timestamp":1573039398000},"page":"5130-5140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Posttranscriptional regulation of MMP\u20109 by HuR contributes to IL\u20101\u03b2\u2010induced pterygium fibroblast migration and invasion"],"prefix":"10.1002","volume":"235","author":[{"given":"Yu\u2010Hong","family":"Cui","sequence":"first","affiliation":[{"name":"Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences Guangzhou Medical University  Guangzhou China"},{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010Yang","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Guangdong Women and Children Hospital  Guangzhou China"},{"name":"Institute of Ophthalmology, School of Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Guangdong No. 2 Provincial People's Hospital  Guangzhou China"},{"name":"Department of Ophthalmology, Guangzhou Red Cross Hospital The Fourth Affiliated Hospital of Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi\u2010Ling","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Public Health and Preventive Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi\u2010Xuan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Public Health and Preventive Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Yi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital Jinan University  Guangzhou China"},{"name":"Institute of Ophthalmology, School of Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Public Health and Preventive Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei\u2010Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital Jinan University  Guangzhou China"},{"name":"Institute of Ophthalmology, School of Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Lin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Public Health and Preventive Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Ophthalmology, School of Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4287-481X","authenticated-orcid":false,"given":"Hong\u2010Wei","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Public Health and Preventive Medicine Jinan University  Guangzhou China"},{"name":"Department of Ophthalmology, The First Affiliated Hospital Jinan University  Guangzhou China"},{"name":"Institute of Ophthalmology, School of Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.9.7.11164"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000150445"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13103"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000854"},{"issue":"12","key":"e_1_2_10_6_1","first-page":"4823","article-title":"Interstitial collagenases as markers of tumor progression","volume":"6","author":"Brinckerhoff C. 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