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Parathyroid hormone (PTH) stimulates the expression of MMP\u201013 via Runx2, a bone transcription factor in rat osteoblastic cells (UMR106\u201001), and histone deacetylase 4 (HDAC4) acts as a corepressor of Runx2. Moreover, microRNAs (miRNAs) play an important role in regulating genes posttranscriptionally. Here, we hypothesized that PTH upregulates the miRNAs targeting HDAC4, which could lead to increased Runx2 activity and MMP\u201013 expression in rat osteoblastic cells. We identified several miRNAs that putatively target rat HDAC4 using bioinformatics tools. miR\u2010873\u20103p was significantly upregulated by PTH in rat osteoblasts. miR\u2010873\u20103p overexpression downregulated HDAC4 protein expression, increased Runx2 binding at the MMP\u201013 promoter, and increased MMP\u201013 messenger RNA expression in UMR106\u201001 cells. A luciferase reporter assay identified the direct targeting of miR\u2010873\u20103p at the 3\u2032\u2010untranslated region of HDAC4. Thus, miR\u2010873\u20103p targeted HDAC4 and relieved the corepressor effect of HDAC4 on Runx2 for MMP\u201013 expression in rat osteoblasts. This study advances our knowledge of posttranscriptional gene regulation occurring in bone and bone\u2010related diseases and clarifies the role of miRNAs as diagnostic biomarkers.<\/jats:p>","DOI":"10.1002\/jcp.29454","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T09:06:50Z","timestamp":1579597610000},"page":"7996-8009","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["miR\u2010873\u20103p targets HDAC4 to stimulate matrix metalloproteinase\u201013 expression upon parathyroid hormone exposure in rat osteoblasts"],"prefix":"10.1002","volume":"235","author":[{"given":"Desai","family":"Malavika","sequence":"first","affiliation":[{"name":"Department of Biotechnology, School of Bioengineering SRM Institute of Science and Technology  Kattankulathur Tamil Nadu 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