{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:22:57Z","timestamp":1770963777896,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T00:00:00Z","timestamp":1579219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672173"],"award-info":[{"award-number":["81672173"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Traumatic osteonecrosis of femoral head (TONFH) is a common orthopedic disease caused by physical injury in hip. However, the unclear pathogenesis mechanism of TONFH and lacking of simple noninvasive early diagnosis method cause the necessity of hip replacement for most patients with TONFH. In this study, we aimed to identify circulating microRNAs (miRNAs) by integrated bioinformatics analyses as potential biomarker of TONFH. mRNA expression profiles were downloaded from the Gene Expression Omnibus database. Then we combined two miRNA screen methods: Weighted gene co\u2010expression network analysis and fold change based differentially expressed miRNAs analysis. As a result, we identified 14 key miRNAs as potential biomarkers for TONFH. Besides, 302 target genes of these miRNAs were obtained and the miRNA\u2013mRNA interaction network was constructed. Furthermore, the results of Kyoto Encyclopedia of Gene and Genome pathway analysis, Gene Ontology function analysis, protein\u2013protein interaction (PPI) network analysis and PPI network module analysis showed close correlation between these 14 key miRNAs and TONFH. Then we established receiver operating characteristic curves and identified 6\u2010miRNA signature with highly diagnosis value including miR\u201093\u20105p (area under the curve [AUC]\u2009=\u20090.93), miR\u20101324 (AUC\u2009=\u20090.92), miR\u20104666a\u20103p (AUC\u2009=\u20090.92), miR\u20105011\u20103p (AUC\u2009=\u20090.92), and miR\u2010320a (AUC\u2009=\u20090.89), miR\u2010185\u20105p (AUC\u2009=\u20090.89). Finally, the results of quantitative real\u2010time polymerase chain reaction confirmed the significantly higher expression of miR\u201093\u20105p and miR\u2010320a in the serum of patients with ONFH. These circulating miRNAs could serve as candidate early diagnosis markers and potential treatment targets of TONFH.<\/jats:p>","DOI":"10.1002\/jcp.29467","type":"journal-article","created":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T10:45:15Z","timestamp":1579257915000},"page":"8129-8140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Identification of potential miRNA biomarkers for traumatic osteonecrosis of femoral head"],"prefix":"10.1002","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1626-5006","authenticated-orcid":false,"given":"Guan\u2010Zhi","family":"Liu","sequence":"first","affiliation":[{"name":"Bone and Joint Surgery Center Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Kong","sequence":"additional","affiliation":[{"name":"Bone and Joint Surgery Center Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Run","family":"Tian","sequence":"additional","affiliation":[{"name":"Bone and Joint Surgery Center Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Bone and Joint Surgery Center Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Li","sequence":"additional","affiliation":[{"name":"Bone and Joint Surgery Center Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an 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