{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:39:11Z","timestamp":1792211951213,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T00:00:00Z","timestamp":1580169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["Ph.D. Thesis no: 95\/4\u20105\/6"],"award-info":[{"award-number":["Ph.D. Thesis no: 95\/4\u20105\/6"]}],"id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cell therapy of damaged tissue, which is linked to hypoxia condition might fail, in large part due to the emergence of oxidative stress (OS) and\/or mitochondrial dysfunctions. Thus, the invigoration of stem cells against oxidative stress could be a reliable strategy to improve the cell therapy outcome. Of various antioxidants, mito\u2010Tempo (mito\u2010T) is one of the potent antioxidants that could target and neutralize the mitochondrial oxidative stress. In this study, for the induction of hypoxia and oxidative stress in mitochondria of the mesenchymal stem cells (MSCs) isolated from human adipose tissue, antimycin A (AMA) was used and then several parameters were analyzed, including cell viability and cell cycle arrest of MSCs exposed to AMA, mito\u2010T, antioxidant potential, redox homeostasis, and signaling pathways in MSCs under oxidative stress. Based on our findings, the treated MSCs were found to impose a high resistance to the OS\u2010induced apoptosis, which correlated with the nuclear factor erythroid 2\u2010related factor 2 (Nrf2) pathway required to manage OS. Upon exposure of the MSCs to high oxidative stress conditions using AMA, the cells failed to scavenge. The use of mito\u2010T was found to alleviate the damage induced by oxidative stress through both direct functions of the free radical scavenging and the interplay in terms of cell signaling pathways including the upregulation of the Nrf2 pathway. These findings may pave the way in the stem cell therapy for the hypoxia\u2010mediated tissue damage.<\/jats:p>","DOI":"10.1002\/jcp.29495","type":"journal-article","created":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T09:13:01Z","timestamp":1580202781000},"page":"5628-5636","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Mitochondria\u2010targeted antioxidant mito\u2010TEMPO alleviate oxidative stress induced by antimycin A in human mesenchymal stem cells"],"prefix":"10.1002","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8429-3618","authenticated-orcid":false,"given":"Abolfazl","family":"Barzegari","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Advanced Medical Sciences Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5367-9956","authenticated-orcid":false,"given":"Mohammad","family":"Nouri","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Advanced Medical Sciences Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Departments of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Gueguen","sequence":"additional","affiliation":[{"name":"INSERM U1148, Laboratory for Vascular Translational Science, Cardiovascular Bioengineering, Universit\u00e9 Paris 13, Sorbonne Paris Cit\u00e9  Villetaneuse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazli","family":"Saeedi","sequence":"additional","affiliation":[{"name":"Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela","family":"Pavon\u2010Djavid","sequence":"additional","affiliation":[{"name":"INSERM U1148, Laboratory for Vascular Translational Science, Cardiovascular Bioengineering, Universit\u00e9 Paris 13, Sorbonne Paris Cit\u00e9  Villetaneuse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadollah","family":"Omidi","sequence":"additional","affiliation":[{"name":"Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Department of Pharmaceutics Faculty of Pharmacy Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.15171\/bi.2017.22"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2014.0484"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.04.001"},{"key":"e_1_2_9_5_1","first-page":"450","article-title":"Oxidative stress and the amyloid beta peptide in Alzheimer's disease. 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