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Bromodomain and extra\u2010terminal domain (BET) family is associated with the pathogenesis of inflammation, and inhibition of BRD4, a vital member of BET family, plays an anti\u2010inflammatory role in many diseases. However, it remains elusive whether BRD4 plays a similar role in nucleus pulposus (NP) cells and participates in the pathogenesis of intervertebral disc degeneration. The present study aims to observe whether BRD4 inhibition regulates matrix metabolism by controlling autophagy and NLRP3 inflammasome activity. Besides, the relationship was investigated among nuclear factor \u03baB (NF\u2010\u03baB) signaling, autophagy and NLRP3 inflammasome in NP cells. Here, real\u2010time polymerase chain reaction, western blot analysis and adenoviral GFP\u2010LC3 vector transduction in vitro were used, and it was revealed that BRD4 inhibition alleviated the matrix degradation and increased autophagy in the presence or absence of tumor necrosis factor \u03b1. Moreover, p65 knockdown or treatment with JQ1 and Bay11\u20107082 demonstrated that BRD4 inhibition attenuated NLRP3 inflammasome activity through NF\u2010\u03baB signaling, while autophagy inhibition by bafilomycin A1 promoted matrix degradation and NLRP3 inflammasome activity in NP cells. In addition, analysis of BRD4 messenger RNA expression in human NP tissues further verified the destructive function of BRD4. Simply, BRD4 inhibition alleviates matrix degradation by enhancing autophagy and suppressing NLRP3 inflammasome activity through NF\u2010\u03baB signaling in NP cells.<\/jats:p>","DOI":"10.1002\/jcp.29508","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T09:35:23Z","timestamp":1579858523000},"page":"5736-5749","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Bromodomain\u2010containing protein 4 inhibition alleviates matrix degradation by enhancing autophagy and suppressing NLRP3 inflammasome activity in NP cells"],"prefix":"10.1002","volume":"235","author":[{"given":"Junmin","family":"Hong","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University Guangzhou Guangdong China"},{"name":"Department of Spine Surgery Sun Yat\u2010sen Memorial 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