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We treated BRL3A rat liver cells with, linoleic (LA), linolenic, oleic (OA), palmitic (PA), palmitoleic (POA), or stearic (SA) acid for 12\u2009hr. The characteristics of cell lipid deposition, oxidative stress indexes, ER stress markers, nuclear factor \u03baB p65 (NF\u2010\u03baB p65), lipid synthesis and transport regulators, and cholesterol metabolism regulators were analyzed. Endoplasmic chaperones like glucose\u2010regulated protein 78, CCAAT\u2010enhancer\u2010binding protein, NF\u2010\u03baB p65, hydrogen peroxide, and malonaldehyde in PA\u2010 and SA\u2010treated cells were significantly higher than in other treated cells. Deposition of fatty acids especially LA and POA were significantly increased than in other treated cells. De novo lipogenesis regulators sterol regulatory element\u2010binding protein 1c, fatty acid synthase, and acetyl\u2010coenzyme A carboxylase 1 (ACC1) expression were significantly increased in all fatty acid stimulation groups, and PA\u2010 and SA\u2010treated cells showed lower p\u2010ACC1 expression and higher scd1 expression than other fatty acid groups. Very low\u2010density lipoprotein synthesis and apolipoprotein B100 expression in free fatty acids treated cells were significantly lower than control. PA, SA, OA, and POA had shown\u00a0significantly increased cholesterol synthesis than other treated cells. PA and SA showed the lower synthesis of cytochrome P7A1 and total bile acids than other fatty acids treated cells. Excess of saturated fatty acids led to severe ER and oxidative stress. Excess unsaturated fatty acids led to increased lipid deposition in cultured hepatocytes. A balanced fatty acid intake is needed to maintain lipid homeostasis.<\/jats:p>","DOI":"10.1002\/jcp.29553","type":"journal-article","created":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T18:24:55Z","timestamp":1580754295000},"page":"6246-6256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Effects of different fatty acids on BRL3A rat liver cell damage"],"prefix":"10.1002","volume":"235","author":[{"given":"Wei","family":"Yang","sequence":"first","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0988-5274","authenticated-orcid":false,"given":"Runqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Xia","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihao","family":"Dong","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoyin","family":"Huang","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruirui","family":"Li","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuang","family":"Xu","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/147323001103900410"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.8.1841"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715762.2015.1078461"},{"issue":"1","key":"e_1_2_9_5_1","first-page":"19","article-title":"Regulation of cholesterol metabolism in liver: Link to NAFLD and Impact of n\u20103 PUFAs","volume":"3","author":"Bae J. 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