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Hyaluronic acid (HA) is recognized by the CD44 receptor, which is overexpressed in many cancer cells. In this study, we developed HA\u2010modified liposomes encapsulating 5\u2010fluorouracil (5\u2010FU) and tested them against a CD44 expressing colorectal cell line (HT29) and a non\u2010CD44 expressing hepatoma cell line. The average size of 5\u2010FU\u2010lipo and 5\u2010FU\u2010lipo\u2010HA nanoparticles were 112\u2009\u00b1\u200928 and 144\u2009\u00b1\u200977\u2009nm, respectively. The 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium (MTT) assay showed selective cancer cell death depending on the CD44 expression in a time\u2010dependent manner. Apoptosis assays and cell\u2010cycle analysis indicated that G0\/G1 arrest occurred. The colony formation study revealed that cells treated with 5\u2010FU\u2010lipo and 5\u2010FU\u2010lipo\u2010HA had reduced colony formation. Quantitative reverse\u2010transcription polymerase chain reaction study showed that the oncogenic messenger RNA and microRNA levels were significantly reduced in the 5\u2010FU\u2010lipo\u2010HA\u2010treated group, while tumor suppressors were increased in that group. We suggest that optimal targeted delivery and release of 5\u2010FU into colorectal cancer cells, renders them susceptible to apoptosis, cell\u2010cycle arrest, and decreased colony formation.<\/jats:p>","DOI":"10.1002\/jcp.29576","type":"journal-article","created":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T09:17:43Z","timestamp":1580203063000},"page":"6817-6830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":115,"title":["Hyaluronic acid\u2010decorated liposomal nanoparticles for targeted delivery of 5\u2010fluorouracil into HT\u201029 colorectal cancer cells"],"prefix":"10.1002","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9444-7134","authenticated-orcid":false,"given":"Behzad","family":"Mansoori","sequence":"first","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Student Research Committee Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Cancer and Inflammation Research, Institute for Molecular Medicine University of Southern Denmark Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4275-628X","authenticated-orcid":false,"given":"Ali","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Cancer and Inflammation Research, Institute for Molecular Medicine University of Southern Denmark Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0134-2021","authenticated-orcid":false,"given":"Fereydoon","family":"Abedi\u2010Gaballu","sequence":"additional","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Biology, Faculty of Natural Sciences University of Tabriz Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7541-1638","authenticated-orcid":false,"given":"Soheil","family":"Abbaspour","sequence":"additional","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2311-4505","authenticated-orcid":false,"given":"Mehri","family":"Ghasabi","sequence":"additional","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6464-7303","authenticated-orcid":false,"given":"Reza","family":"Yekta","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Natural Sciences University of Tabriz Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2303-8645","authenticated-orcid":false,"given":"Solmaz","family":"Shirjang","sequence":"additional","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7813-5226","authenticated-orcid":false,"given":"Gholamreza","family":"Dehghan","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Natural Sciences University of Tabriz Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6431-4605","authenticated-orcid":false,"given":"Michael R.","family":"Hamblin","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine Massachusetts General Hospital Boston Massachusetts"},{"name":"Department of Dermatology Harvard Medical School Boston Massachusetts"},{"name":"Harvard\u2010MIT Division of Health Sciences and Technology Cambridge Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8642-6795","authenticated-orcid":false,"given":"Behzad","family":"Baradaran","sequence":"additional","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2018.05.002"},{"issue":"12","key":"e_1_2_9_3_1","first-page":"4750","article-title":"High affinity restricts the localization and tumor penetration of single\u2010chain fv antibody molecules","volume":"61","author":"Adams G. 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