{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T10:13:25Z","timestamp":1787998405767,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T00:00:00Z","timestamp":1580860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100015034","name":"Shahid Sadoughi University of Medical Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100015034","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inflammatory cytokine, interleukin\u20106 (IL\u20106), plays an important role in the pathogenesis of cardiac hypertrophy. Recent studies have documented that resveratrol exhibits cardioprotective effects. The present study attempts to explore whether resveratrol suppreses IL\u20106 in hypertrophied H9c2 cardiomyoblasts through histone deacetylase, sirtuin 1 (SIRT1). To induce hypertrophy, the cells were incubated with angiotensin II (Ang II). Treatment groups were treated with different doses (1, 10, 25, 50, 75, and 100\u2009\u03bcM) of resveratrol (R). Cell viability was measured using 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide assay. Cell size was determined using crystal violet staining. Gene expression was assessed by real\u2010time polymerase chain reaction technique. Enzyme\u2010linked immunosorbent assay was used to measure IL\u20106 concentration. The results showed that cell area and ANP messenger RNA (mRNA) levels decreased significantly in R25+Ang, R50+Ang, and R100+Ang groups, as compared with Ang group. Therefore, 10, 20, 30, 40, and 50\u2009\u03bcM of resveratrol were used to to evaluate its anti\u2010inflammatory effects. The results revealed that Ang II upregulated IL\u20106 at both mRNA and protein levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001 vs. normal) and resveratrol (50\u2009\u03bcM) decreased IL\u20106 mRNA (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) and protein (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) significantly in comparison to Ang group. However, in groups in which the cells were pretreated with SIRT1 inhibitor, EX\u2010527, the response of resveratrol was partially reversed. Transcription levels of IL\u20106 receptor components (gp130 and gp80) did not change significantly among the experimental groups. The current data suggests that resveratrol protects H9c2 cells against Ang II\u2010induced hypertrophy by suppression of IL\u20106 through SIRT1 activation.<\/jats:p>","DOI":"10.1002\/jcp.29592","type":"journal-article","created":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T04:21:26Z","timestamp":1580962886000},"page":"6969-6977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Resveratrol suppresses interleukin\u20106 expression through activation of sirtuin 1 in hypertrophied H9c2 cardiomyoblasts"],"prefix":"10.1002","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2738-4940","authenticated-orcid":false,"given":"Fariba","family":"Akhondzadeh","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Shahid Sadoughi University of Medical Sciences  Yazd 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