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In this study, pig oocytes recovered from SF of 1\u20132\u2009mm and medium\u2010follicles (MF) of 3\u20136\u2009mm in diameter from abattoir ovaries were treated by various MAM treatments to improve their competence. The results showed that 25\u2009\u00b5M roscovitine or 1\u2009mM db\u2010cAMP efficiently blocked germinal vesicle breakdown in both SF and MF oocytes suggesting a similar cyclin\u2010dependent kinase (CDK) 1 level between the two oocyte groups. MAM with 15\u2010 and 25\u2010\u00b5M roscovitine alone or with 1\u2010mM db\u2010cAMP improved competence of SF and MF oocytes, respectively, with a promoted chromatin configuration transition from surrounded nucleoli (SN) to re\u2010decondensation (RDC) pattern that supported substantial gene transcription. However, MAM with db\u2010cAMP alone or with higher concentrations of roscovitine did not improve oocyte competence, could not support an SN\u2010to\u2010RDC transition, and\/or evoked a premature chromatin condensation (PMC) that suppressed gene transcription. Both CDK2 and CDK5 contents were higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) in MF than in SF oocytes. It is concluded that the competence of pig oocytes, particularly that of SF oocytes can be improved by MAM using a proper roscovitine concentration that promotes gene transcription by inhibiting CDK5 while letting CDK2 off to prevent PMC.<\/jats:p>","DOI":"10.1002\/jcp.29655","type":"journal-article","created":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T11:46:14Z","timestamp":1582717574000},"page":"7530-7540","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Meiotic block with roscovitine improves competence of porcine oocytes by fine\u2010tuning activities of different cyclin\u2010dependent kinases"],"prefix":"10.1002","volume":"235","author":[{"given":"Shuai","family":"Zhu","sequence":"first","affiliation":[{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention College of Animal Science and Veterinary Medicine  Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying\u2010Jun","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Life Science North Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu\u2010Zhu","family":"Pan","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention College of Animal Science and Veterinary Medicine  Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Gong","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention College of Animal Science and Veterinary Medicine  Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Ju","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Life Science North Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo\u2010Liang","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention College of Animal Science and Veterinary Medicine  Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Jiu","family":"Luo","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention College of Animal Science and Veterinary Medicine  Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6437-6259","authenticated-orcid":false,"given":"Jing\u2010He","family":"Tan","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention College of Animal Science and Veterinary Medicine  Tai'an China"},{"name":"College of Life Science North Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199812)51:4<395::AID-MRD6>3.0.CO;2-Y"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds061"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199404002771"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200409055"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.0518a.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01924.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16119-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00534.2009"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00352-9"},{"key":"e_1_2_10_11_1","first-page":"125","volume-title":"Beltsville symposia in agricultural research XX. 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