{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T05:48:34Z","timestamp":1766382514925,"version":"3.37.3"},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T00:00:00Z","timestamp":1585785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Start-up Foundation for Doctoral research of Guangdong Medical University","award":["B2019004"],"award-info":[{"award-number":["B2019004"]}]},{"name":"Medical Scientific Research Foundation of Guangdong Province","award":["A2019335"],"award-info":[{"award-number":["A2019335"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the regulation of Cl<jats:sup>\u2212<\/jats:sup> secretion by adrenoceptors in polarized 16HBE14o\u2010 human bronchial epithelial cells. Treatment with the nonselective \u03b2 adrenoceptor agonist isoprenaline stimulated an increase in short\u2010circuit current (<jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub>), which was inhibited by the \u03b2 adrenoceptor blocker propranolol. Treatment with procaterol, an agonist specific for the \u03b2<jats:sub>2<\/jats:sub> adrenoceptor subtype, stimulated a similar increase in <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub>, which was inhibited by the \u03b2<jats:sub>2<\/jats:sub> adrenoceptor antagonist ICI 118551. Inhibitors of cystic fibrosis transmembrane conductance regulator (CFTR) and calcium\u2010activated Cl<jats:sup>\u2212<\/jats:sup> channel (CaCC), but not K<jats:sup>+<\/jats:sup> channel blockers, were able to inhibit the increase in <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub>. \u201cTrimultaneous\u201d recording of <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> and intracellular cyclic adenosine monophosphate (cAMP) and Ca<jats:sup>2+<\/jats:sup> levels in 16HBE14o\u2010 epithelia confirmed that the <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> induced by isoprenaline or procaterol involved both cAMP and Ca<jats:sup>2+<\/jats:sup> signaling. Our results demonstrate that \u03b2<jats:sub>2<\/jats:sub> adrenoceptors regulate Cl<jats:sup>\u2212<\/jats:sup> secretion in the human airway epithelium by activating apical CFTRs and CaCCs via cAMP\u2010dependent and intracellular Ca<jats:sup>2+<\/jats:sup>\u2010dependent mechanisms, respectively.<\/jats:p>","DOI":"10.1002\/jcp.29683","type":"journal-article","created":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T11:53:10Z","timestamp":1585828390000},"page":"8387-8401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["\u03b2<sub>2<\/sub> adrenoceptor signaling regulates ion transport in 16HBE14o\u2010 human airway epithelial cells"],"prefix":"10.1002","volume":"235","author":[{"given":"Rui\u2010Gang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Physiology Basic Medical School, Guangdong Medical University  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